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Model taste cells and methods of use for identifying modulators of taste sensation

A taste cell and pattern technology, applied in receptors/cell surface antigens/cell surface determinants, specific peptides, instruments, etc., can solve the problems of lack of knowledge and limitations of taste cell proteins

Inactive Publication Date: 2009-07-29
THE COCA COLA CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of new agents that mimic or block the four basic tastes has been limited by a lack of understanding of the taste cell proteins responsible for transducing taste patterns

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 produces the method for pattern taste cell

[0055] [052] Human HuTu-80 endocrine cells were prepared according to the method described by Rozengurt et al. (2006, Am J Physiol Gastrointest Liver Physiol 291:792-802). 5% CO at 37°C 2 / 95% air conditions, the parent HuTu-80 (ATCC: HTB-40 TM ) Endocrine cells were grown in Minimal Essential Eagle's Medium containing 10% FBS and antibiotics (100 U / ml penicillin, 100 μg / ml streptomycin and 0.25 μg / ml amphotericin B) in plastic or collagen I-coated plates .

Embodiment 2

[0056] Example 2 Using Model Taste Cells to Screen and Identify Modulators of Taste Signal Transduction

[0057] [053] One or more target proteins necessary for taste signal transduction are isolated and purified from model taste cells as described above. The effect of test compounds on purified target proteins or their interaction with other proteins in taste signal transduction is determined using various cell-based assays as described below. Further confirmation that the test compounds modulate taste signaling is then identified based on the cell-based assays performed to identify test compounds that modulate the purified protein of interest or their interaction with other proteins in taste signaling.

[0058] [054] As used herein, cell-based assays include:

[0059] A. Cell-based analysis of intracellular second messengers:

[0060] [055] Detection of cyclic nucleotides: Changes in cyclic nucleotides such as cAMP and cGMP can be measured by quantifying their amounts in ...

Embodiment 3

[0079] Confirmation of the Effects of Taste Modulating Compounds in the Human Taste Test of Example 3

[0080] [071] Test tastants (e.g., sweeteners, savory compounds, salty tastants, bitter tastants, or sour tastants) tasted alone in combination with test tastants and taste-modulating compounds Perceived intensity for comparison. A candidate taste enhancer increases the perceived intensity of a test tastant, while a taste suppressor decreases the perceived intensity of the test tastant.

[0081] [072] In one embodiment, a panel of assessors is used to measure the strength of the test tastant solution. Briefly, a group of evaluators (usually 8 to 12 individuals) are trained to evaluate taste intensity perception and intensity is measured at several time points from the time the sample first enters the mouth until 3 minutes after it has been expelled. Using statistical analysis, the results were compared between samples containing additives and samples not containing additive...

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PUM

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Abstract

The present invention provides model taste cells that naturally or recombinant Iy express taste receptors and relevant cellular proteins and / or molecules useful for taste signal transduction. The present invention further provides methods of use for these model taste cells for screening for compounds that modulate sweet and / or other taste signal transduction. Compositions comprising the compounds / modulators identified using the model taste cells are also provided. In preferred embodiments, the model taste cells are derived from human HuTu-80 enteroendocrine cells, and derivative cells thereof.

Description

[0001] Cross References to Related Applications [0002] [001] This application claims priority to US Provisional Application Serial No. 60 / 820,490, filed July 27, 2006, the entire contents of which are hereby incorporated by reference. field of invention [0003] [002] The present invention relates to cells endogenously and / or naturally expressing one or more signaling proteins and associated cellular machinery necessary for taste signal transduction, and the use of these cells to identify compounds that modulate taste signal transduction / modulator method. In particular, the present invention relates to methods for identifying taste modulating compounds using HuTu-80 human enteroendocrine cells and cells derived therefrom, which naturally express taste receptors and associated cell signaling machinery. Background of the invention [0004] [003] Taste cells are organized into taste buds on the surface of the tongue (Lindemann, 1996, Physol. Rev. 76:718-66). Two families o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/705G01N33/566G01N33/53
CPCG01N33/5041G01N2333/726
Inventor 哈里什·拉达克瑞施那迈克尔·D·布朗
Owner THE COCA COLA CO
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