Method for extracting veratrum alkaloid from Veratrum nigrum by supercritical carbon dioxide

A carbon dioxide and supercritical technology, which is applied in the field of extraction of active ingredients of natural plants, can solve the problems of large consumption of organic solvents and long extraction time, and achieve the effect of simple process and stable operation

Inactive Publication Date: 2009-08-12
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent No. 200610170802 discloses a process for extracting veratrum alkaloids from veratrum. The above method is used. This method takes a long time to extract and consumes a lot of organic solvents

Method used

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  • Method for extracting veratrum alkaloid from Veratrum nigrum by supercritical carbon dioxide

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Embodiment 1, the method that supercritical carbon dioxide of the present invention extracts veratrum alkaloids from veratrum

[0013] A, pulverize the veratrum, soak for 3 hours according to the ratio of adding 150ml of ammonia water per 100g of veratrum, and pass through a 30-mesh sieve after drying in a cool and ventilated place;

[0014] B. Add to the supercritical fluid extraction tank, open the CO 2 Steel cylinder, after the gas passes through the purifier I, it enters the condenser for liquefaction, then pressurizes with a plunger pump and then enters the purifier II for further liquefaction, and enters the bottom of the extractor through the pipeline. Set the extractor pressure to 35MPa, temperature to 65°C, and static extraction to 0.5 hours, using CO 2 The high-pressure, high-osmotic power makes the alkaloids in veratrum separate, and the active ingredients are dissolved; then, start the entrainer pump and inject 95% ethanol at the same time, and extract dyna...

Embodiment 2

[0017] Embodiment 2, Veratrum direct supercritical carbon dioxide extraction without alkalization treatment

[0018] A, the veratrum is pulverized, crosses 30 mesh sieves;

[0019] B, the extraction method with embodiment 1.

[0020] The crude alkaloid yield of Veratrum is 1.91%-2.42%, and the alkaloid content is 1.18%-2.34%.

Embodiment 3

[0021] Embodiment 3, Veratrum is treated with ethanol and then carried out supercritical carbon dioxide extraction

[0022] A, take by weighing 100g of Veratrum pulverized product, extract 3 times with 95% ethanol 35 ℃ of constant temperature oscillations of 1000ml (share 3000ml ethanol), extract 24 hours each time;

[0023] B, the extraction method with embodiment 1.

[0024] The crude alkaloid yield of Veratrum is 8.53%-10.53%, and the alkaloid content is 4.53%-5.27%.

[0025] The veratrum alkaloid yield that is measured by contrasting examples 1,2,3 illustrates that the present invention adopts supercritical CO after pre-alkalization 2 The method for extracting veratrum alkaloids from veratrum is higher than the organic solvent extraction method, and greatly reduces the amount of organic solvent used and shortens the time.

[0026] The supercritical CO of the present invention 2 The extraction equipment was selected from Jiangsu Nantong Huaan Supercritical Extraction Co....

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Abstract

The invention provides a method for extracting veratrum alkaloid from veratrum by supercritical carbon dioxide, which comprises the following steps of: A. crushing the veratrum, adding 150mL of ammonia into 100g of veratrum powder and soaking for 3 hours, drying at a shade and ventilating place and then sieving with 30 meshes; B. adding the crushed product of the veratrum after alkalized into a supercritical fluid extracting tank, opening a CO2 steel cylinder, starting to extract by taking ethanol as a carrying agent at the temperature of an extractor of 65 to 75 DEG C, at the extracting pressure of 30 to 38 MPa, at the flow rate of CO2 of 40 to 45Kg / h and with the extracting time of 2 to 4 hours, and implementing distillation by a separator and recovering a solvent so as to obtain crude products of veratrum alkaloid. The invention adopts early-phase alkaline treatment, extracts veratrum alkaloid from veratrum by supercritical carbon dioxide, can separate the combined alkaloid and acid which are hard to separate from each other in veratrum by utilizing high pressure and high seepage pressure of CO2, and dissolves out effective ingredients, the yield of the veratrum alkaloid is higher than that of veratrum alkaloid extracted by soaking of organic solvent; in addition, the invention has simple process, stable operation and recycled solvent and has a certain economic benefit and environmental benefit.

Description

technical field [0001] The invention relates to a method for extracting veratrum alkaloids from veratrum, and belongs to the technical field of extraction methods of natural plant active ingredients. Background technique [0002] Veratrum alkaloid is an important biologically active substance naturally present in Veratrum, which has many important physiological functions and great development and application value. It has contact killing and stomach poisoning effects on a variety of insects, and can develop biological pesticides; small doses of veratrum alkaloids have therapeutic effects on high blood pressure, stroke, epilepsy, and fractures, and can be developed into health care products and medicines. One of the most concerned natural compounds. At present, the method for preparing veratrum alkaloids is to extract the crude extract of veratrum alkaloids from veratrum by using the traditional organic solvent soaking extraction method at home and abroad. Patent No. 200610...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A01N65/42A61P9/12A61P25/08A61P19/08A61P9/10A01P7/04
Inventor 魏福祥王占辉
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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