Preparation of microbial oligosaccharide with prebiotic function

A technology of microorganisms and prebiotics, which is applied in the field of preparation of microbial oligosaccharides, and can solve problems such as microbial oligosaccharides that have not yet been seen

Inactive Publication Date: 2009-08-12
OCEAN UNIV OF CHINA
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been studies on the use of chitosan oligosaccharides, fructooligosaccharides, mannan oligosaccharides, xylooligosacchari

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of microbial oligosaccharide with prebiotic function
  • Preparation of microbial oligosaccharide with prebiotic function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0008] Prepare the sugar production medium first, weigh 1.0g of yeast extract, 1.0g of peptone, 30g of glucose, Na 2 HPO 4 10g, KH 2 PO 4 ·3H 2 O 3g, MgSO 4 ·7H 2 O 0.2g, NaCl 1.0g, K 2 SO 4 10g, CaCl 2 0.001g, and finally add water to a total weight of 1kg. Then the pH of the enzyme production medium was adjusted to 7.2 with 1M NaOH aqueous solution. The enzyme-producing medium was sterilized by high-pressure steam at 112°C for 20 minutes, and then cooled to room temperature. Klebsiella K13 was cultured in liquid shaking with ordinary bacterial culture medium, and the growth reached the logarithmic phase after 24 hours. The culture liquid at this time was inoculated into 200ml of the above-mentioned sugar-producing medium as fermentation seeds, and liquid fermentation was carried out at 30°C for 120 hours, the fermentation broth was centrifuged to remove precipitated cells and proteins, the supernatant was collected and concentrated to 40ml by rotary evaporation ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing microbial oligosaccharide with probiotic action. The method is characterized in that: a sugar producing culture medium is used for liquid fermentation of K.rhinoscleromatis K13 to extract extracellular polysaccharide; enzymatic solution of phage glycanase is prepared, the enzymatic solution and water solution of extracellular polysaccharide are mixed for enzymolysis, the undegraded macro molecular sugar is removed by ultrafiltration, and the obtained filtrate is refined and condensed to obtain the microbial oligosaccharide KOS. Tests show that the microbial oligosaccharide KOS is non-digestible oligosaccharide which can promote the growth of probiotics such as bifidobacterium, lactobacillus and the like, inhibit the growth of harmful bacteria such as pathogenic bacteria, putrefying bacteria and the like, and has the functions of improving the micro ecological environment of the intestinal tract and cleaning the intestinal tract. The microbial oligosaccharide KOS is novel probiotics, which is novel microbial oligosaccharide which is obtained by degrading the extracellular polysaccharide of the K.rhinoscleromatis K13 through the phage glycanase, and the raw materials used in the method are different from those of other polysaccharides, and the result repeatability is good due to the degradation by an enzyme method, so that the application prospect is wide.

Description

technical field [0001] The invention relates to a preparation method of microbial oligosaccharide with prebiotic effect. Background technique [0002] Prebiotics refer to a non-digestible food functional factor that can selectively promote the growth or activity of one or a limited number of beneficial bacteria (probiotics) in the intestinal tract of the body. An oligosaccharide or oligosaccharide. In the 1990s, Gibson first proposed the concept of prebiotics, and now prebiotics have received extensive attention and research as a new type of microecological regulator. Due to their structural specificity, prebiotics are neither digested and absorbed by the human digestive system, nor decomposed and utilized by harmful intestinal flora, but can only be utilized by beneficial intestinal flora such as bifidobacteria and lactic acid bacteria, thereby achieving adjustment The purpose of normal intestinal flora. At present, there have been studies on the use of chitosan oligosac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/00C12R1/22
Inventor 牟海津乔来艳王鹏
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap