Preparation of microbial oligosaccharide with prebiotic function

Abstract

The invention provides a method for preparing microbial oligosaccharide with probiotic action. The method is characterized in that: a sugar producing culture medium is used for liquid fermentation of K.rhinoscleromatis K13 to extract extracellular polysaccharide; enzymatic solution of phage glycanase is prepared, the enzymatic solution and water solution of extracellular polysaccharide are mixed for enzymolysis, the undegraded macro molecular sugar is removed by ultrafiltration, and the obtained filtrate is refined and condensed to obtain the microbial oligosaccharide KOS. Tests show that the microbial oligosaccharide KOS is non-digestible oligosaccharide which can promote the growth of probiotics such as bifidobacterium, lactobacillus and the like, inhibit the growth of harmful bacteria such as pathogenic bacteria, putrefying bacteria and the like, and has the functions of improving the micro ecological environment of the intestinal tract and cleaning the intestinal tract. The microbial oligosaccharide KOS is novel probiotics, which is novel microbial oligosaccharide which is obtained by degrading the extracellular polysaccharide of the K.rhinoscleromatis K13 through the phage glycanase, and the raw materials used in the method are different from those of other polysaccharides, and the result repeatability is good due to the degradation by an enzyme method, so that the application prospect is wide.

Description

technical field [0001] The invention relates to a preparation method of microbial oligosaccharide with prebiotic effect. Background technique [0002] Prebiotics refer to a non-digestible food functional factor that can selectively promote the growth or activity of one or a limited number of beneficial bacteria (probiotics) in the intestinal tract of the body. An oligosaccharide or oligosaccharide. In the 1990s, Gibson first proposed the concept of prebiotics, and now prebiotics have received extensive attention and research as a new type of microecological regulator. Due to their structural specificity, prebiotics are neither digested and absorbed by the human digestive system, nor decomposed and utilized by harmful intestinal flora, but can only be utilized by beneficial intestinal flora such as bifidobacteria and lactic acid bacteria, thereby achieving adjustment The purpose of normal intestinal flora. At present, there have been studies on the use of chitosan oligosac...

AI Technical Summary

Problems solved by technology

At present, there have been studies on the use of chitosan oligosaccharides, fructooligosaccharides, mannan oligosaccharides, xylooligosaccharides, agar oligosaccharides, etc. as prebiotics at home and abroad, but there is no report on the use of microbial oligosaccharides as prebiotics

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