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Method by adopting complex enzyme to prepare rice residue protein

A technology of rice dregs protein and compound enzyme, applied in the direction of plant protein processing, can solve the problems of low purity and extraction rate of rice dregs protein, difficult industrial production, difficult waste water and harmful substances, etc., to reduce the difficulty of wastewater treatment and production. The effect of cost reduction and environmental protection

Inactive Publication Date: 2010-12-29
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the alkali leaching method is concerned, the concentration of alkali is generally about 1wt%, and the alkali is strong. Its biggest disadvantage is that the prepared rice dregs protein has low purity and extraction rate, and it is easy to produce toxic peptides; meanwhile, due to a large amount of waste water and harmful substances are difficult to handle, causing serious environmental pollution
The purity of protein extracted by protease method is good, but the extraction rate is also very low, so it is difficult to apply to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Pass the rice dregs raw material through an 80-mesh sieve, weigh 100g of the sieved rice dregs, add 1kg of drinking tap water, stir and infiltrate for 14 minutes; (2) season the pH value of the water with a concentration of 0.1mol / L hydrochloric acid solution to 5.0, add 15U / g cellulase according to the mass of raw rice dregs, keep warm at 50°C, stir continuously for 1 hour, centrifuge, discard the supernatant; repeat this process twice to get most of the dextrin and oligosaccharides (3) Add 1 kg of water to the protein wet base after cellulase treatment, adjust the pH to 4.5 with hydrochloric acid solution, and then add 40 U / g of amylase (according to raw material rice dregs mass calculation), stirred at 60°C for 1 hour, centrifuged, and discarded the supernatant to obtain the amylase-treated wet protein; (4) add 1 kg of water to the enzyme-treated wet starch and stir at 90°C to kill The enzyme is separated by centrifugation, and the supernatant is discarded, and t...

Embodiment 2

[0014] (1) pass the rice dregs raw material through a 160 mesh sieve, weigh 100g of the sieved rice dregs, add 800g of drinking tap water, stir and infiltrate for 10 minutes; (2) season the pH value of the water with a concentration of 0.08mol / L hydrochloric acid solution to 6.0, add 20U / g cellulase according to the mass of raw rice dregs, keep warm at 40°C, keep stirring for 2 hours, centrifuge, discard the supernatant; repeat this process twice to get most of the dextrin and oligosaccharides (3) Add 800g of water to the protein wet base after cellulase treatment, adjust the pH to 5.5 with hydrochloric acid solution, and then add 50U / g of amylase (according to raw material rice dregs mass calculation), stirred at 50°C for 1.5 hours, centrifuged, and discarded the supernatant to obtain the amylase-treated wet protein; (4) add 700g of water to the enzyme-treated wet starch and stir at 85°C The enzyme is separated by centrifugation, and the supernatant is discarded, and this pro...

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PUM

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Abstract

The invention relates to a method by adopting complex enzyme to prepare rice residue protein. The method comprises the following steps: (1) pre-treatment of rice residue materials; (2) cellulose enzyme treatment; (3) amylase treatment; and (4) enzyme inactivation and drying. The rice residue protein prepared by the invention has good quality; the content of dextrinose and oligosaccharide are lower than 5wt percent; the content of protein is larger than 90wt percent; compared with an alkali steeping method and a protease method, the waste water is easy to be treated, thus being beneficial to protecting environment; and compared with the protease method, the production cost is reduced greatly.

Description

technical field [0001] The invention relates to a method for preparing protein, in particular to a method for preparing rice dreg protein. Background technique [0002] Rice dregs are the leftovers after making starch sugar or monosodium glutamate from rice, and the protein content is as high as more than 60% (dry basis). In the past, it was mostly discharged with waste liquid, which not only caused waste of resources, but also polluted the environment; at present, it is mostly used as feed, and its industrial added value is low. [0003] In fact, rice dregs protein belongs to high-quality grain protein, has a good amino acid composition ratio, contains eight kinds of amino acids necessary for the human body, and has a wide range of raw materials and low prices, and has extremely important comprehensive recycling value. In terms of its protein content, source and price, it is an ideal raw material for extracting rice protein. [0004] However, due to the high temperature t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/34A23J3/14
Inventor 田蔚林亲录
Owner HUNAN AGRICULTURAL UNIV