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Manufacturing method of paraffin tissue chip

A production method and tissue chip technology, which are applied in the preparation of test samples, material inspection products, biological testing, etc., can solve the problems of limited tissue thickness, tiny tissue, and limited tissue sources, and achieve high yield and diverse materials.

Inactive Publication Date: 2009-10-14
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the preparation of tissue chips according to the above-mentioned method has the following disadvantages at present: first, the production cost of existing tissue chips is very high
In addition to special equipment (hundreds of thousands of yuan), the cost of making a tissue chip is more than 10,000 yuan, and the unit price of each slice is 50-100 yuan; The source is limited; thirdly, the chip prepared according to the existing equipment and method has only about 50 slices, because the sample is made by punching holes on the original wax block, and the tissue thickness is limited; fourthly, although the high-density chip has a large throughput, However, due to the small size of each tissue, it is difficult to provide information on the tissue structure; fifth, only in large-scale research units, there is funding and conditions for tissue chip-related research, which is difficult to promote

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Paraffin tissue chips were prepared by the following method:

[0021] Step 1: Take the required tissues when collecting pathological materials, number them, cut them into a size of 2mm×2mm×10mm, follow the routine tissue processing procedures of the pathology department, and go to the step of soaking them in paraffin; put them on the ironing table at 60°C The paraffin on the surface is melted to obtain the tissue to be embedded;

[0022] Step 2: Take another paraffin and put it into a paraffin pot, and heat it to 60°C with an electric furnace to melt the paraffin;

[0023] Step 3: Put 2 vertically bent copper strips on a thick glass plate, put together a square wax mold with a length of 70mm, a width of 40mm, a height of 20mm, and a thickness of 10mm on a glass plate, with a thickness of (copper The height of the strip), use tweezers to take the first tissue to be embedded, quickly immerse it once in melted paraffin, stick the flat surface to the glass, rely on the sti...

Embodiment 2

[0025] Paraffin tissue chips were prepared by the following method:

[0026] Step 1: Number and position the required tissue on the wax block, cut the tissue into a square block with a length of 5mm, a width of 1mm, and a thickness of 1mm; put it on the ironing table, and melt the paraffin on it at 65°C Obtain the tissue to be embedded;

[0027] Step 2: Take another paraffin and put it into a paraffin pot, and heat it to 70°C with an electric furnace to melt the paraffin;

[0028] Step 3: Put 2 vertically bent copper strips on a thick glass plate, put together a square wax mold with a length of 70mm, a width of 40mm, a height of 20mm, and a thickness of 20mm on a glass plate, with a thickness of (copper The height of the strip), use tweezers to take the first tissue to be embedded, quickly immerse it once in melted paraffin, stick the flat surface to the glass, rely on the stickiness of the paraffin to stand the tissue on the glass, the first tissue has The two sides are clo...

Embodiment 3

[0030] Paraffin tissue chips were prepared by the following method:

[0031] Step 1: Number and position the required tissue on the wax block, cut the tissue into a cylinder with a length of 10mm and a radius of 1mm; put it on the ironing table, and melt the paraffin on it at 65°C to obtain the tissue to be embedded organization;

[0032] Step 2: Take another paraffin and put it into a paraffin pot, and heat it to 70°C with an electric furnace to melt the paraffin;

[0033]Step 3: Put 2 vertically bent copper strips on a thick glass plate, put together a square wax mold with a length of 70mm, a width of 40mm, a height of 20mm, and a thickness of 20mm on a glass plate, with a thickness of (copper The height of the strip), use tweezers to take the first piece of tissue to be embedded, quickly immerse it once in melted paraffin, stick the flat surface to the glass, rely on the stickiness of the paraffin to stand the tissue on the glass, the first piece of tissue has two The sid...

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Abstract

The invention provides a manufacturing method of a paraffin tissue chip, which is characterized by comprising the following detailed steps of: numbering the tissues needed, then impregnating paraffin or numbering after marking the tissues needed on the existing paraffin; cutting the tissues into needed size, putting the cut tissues on an ironing table, melting the paraffin thereof at the temperature of 60 to 65 DEG C to obtain tissues to be embedded; taking another paraffin into a paraffin pot, and using an electric furnace to heat and melt the paraffin; putting a square paraffin mold on a glass board, impregnating the tissues to be embedded obtained by the step 1 in the melten paraffin, arranging the tissues at one side of the inner part of the paraffin mold, recording the numbers of tissues of corresponding positions on a positioning board or a positioning paper, reserving a square vacant position at the other side of the inner part of the paraffin mold, pouring the melten paraffin into the paraffin mold along the vacant position till the tissues are impregnated, polymerizing the paraffin, removing the paraffin mold and cutting the obtained paraffin into pieces along the direction parallel to the glass board. The manufacturing method of the paraffin tissue chip has the advantages of cheap price, convenience, fastness, high yield and diverse material-obtaining and can be popularized into each pathology department and relevant research units.

Description

technical field [0001] The invention relates to a method for preparing a paraffin tissue chip, which is used for preparing a tissue chip and belongs to the technical field of tissue chips. Background technique [0002] Tissue chip technology is the development and extension of gene chip technology. Like cell chip, protein chip and antibody chip, it belongs to a special biochip technology. Tissue chip technology can arrange dozens or even thousands of clinical tissue specimens of different individuals in a pre-designed sequence for analysis and research. It is a high-throughput, multi-sample analysis tool. Tissue chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can be combined with DNA, RNA, protein, antibody and other technologies, combined with traditional pathology technology, histochemistry and immunohistoch...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/48
Inventor 侯英勇宿杰·阿克苏曾海英谭云山
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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