Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same
A technology for simulating particles and white blood cells, applied in the field of blood cell analysis, can solve the problems of complex white blood cells, increased process difficulty, and increased cost
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Embodiment 1
[0101] Example 1: Simulating lymphocytes with human erythrocytes
[0102] 1. Configure the following treatment reagents: add 1.5g sodium chloride, 1g sodium nitrite, 100μl of 1% (w / v) sodium hexadecylsulfonate and 100μl 25% (v / v) pentane to 195ml pure water Dialdehyde;
[0103] 2. Mix 5ml of human anticoagulated whole blood with the above-mentioned treatment reagent, and let it stand at room temperature for about 30 minutes;
[0104] 3. Measure on the computer (BC5500), and determine whether to start to strengthen the fixation according to different distribution requirements;
[0105] 4. When the reactants meet the required distribution requirements (that is, when the DIFF image is located in the lymphocyte area), add a 1:10 glutaraldehyde-formaldehyde mixture with a final concentration of 0.5% (v / v) in the reaction solution , placed at room temperature for 2 hours.
[0106] 5. Measure on the machine, observe the changes in counting and cell distribution;
[0107] 6. After...
Embodiment 2
[0108] Example 2: Simulating neutrophils with elephant erythrocytes
[0109] 1. Configure the following treatment reagents: add 1.5g sodium chloride, 1g sodium nitrite, 100μl of 1% (w / v) sodium hexadecylsulfonate and 100μl 25% (v / v) pentane to 195ml pure water Dialdehyde;
[0110] 2. Mix 5ml anticoagulant whole blood and processing reagents, and place at room temperature for about 30 minutes;
[0111] 3. Test on the computer, and determine whether to start strengthening the fixation according to different distribution requirements;
[0112] 4. When the reactant meets the required distribution requirements (that is, when the DIFF image is already in the neutrophil area), add 1:10 glutaraldehyde-formaldehyde with a final concentration of 0.5% (v / v) to the reaction solution The mixture was left at room temperature for 2 hours.
[0113] 5. On the machine (BC5500) to measure, observe the changes in count and cell distribution;
[0114] 6. After the requirements are met (that is...
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