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Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same

A technology for simulating particles and white blood cells, applied in the field of blood cell analysis, can solve the problems of complex white blood cells, increased process difficulty, and increased cost

Active Publication Date: 2013-08-21
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) It is difficult to obtain white blood cell particles, which directly leads to increased cost and complicated process
[0006] In the blood of humans and animals, the concentration of white blood cells is only 1 / 100-1 / 1000 of that of red blood cells, and the process of obtaining white blood cells without red blood cells is very complicated
However, the use of hemolytic agents may lead to changes in the characteristics of leukocytes, so that the physiological leukocyte state can no longer be used to evaluate the purified leukocyte particles
In addition, the process of removing red blood cells is very complicated, and it is not easy to achieve satisfactory results even if high-cost cell separation fluid is used, so the cost and products of the calibrator and quality control materials of the five-differential blood cell analyzer are greatly limited; and
[0007] (2) It is difficult to adopt enhanced aldehyde fixation technology using white blood cells, resulting in insufficient stability of the product
[0012] (2) The processing technology of the red blood cell material disclosed in the prior art is relatively complicated, and volume adjustment (including stability pretreatment before adjustment), adjustment of hemoglobin content and long-term low-temperature fixation are required. Due to the complicated processing links, especially Volume expansion regulation greatly reduces the integrity of the red blood cell membrane, resulting in problems with its stability and yield
In addition, too many processing steps make the process more difficult, and the repeatability of the product will also be affected to a certain extent, which will reduce the practicability and controllability of the process

Method used

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  • Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same
  • Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same
  • Five-classification leucocyte simulacrum particle, method for preparing same, and quality control substance and calibration substance containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1: Simulating lymphocytes with human erythrocytes

[0102] 1. Configure the following treatment reagents: add 1.5g sodium chloride, 1g sodium nitrite, 100μl of 1% (w / v) sodium hexadecylsulfonate and 100μl 25% (v / v) pentane to 195ml pure water Dialdehyde;

[0103] 2. Mix 5ml of human anticoagulated whole blood with the above-mentioned treatment reagent, and let it stand at room temperature for about 30 minutes;

[0104] 3. Measure on the computer (BC5500), and determine whether to start to strengthen the fixation according to different distribution requirements;

[0105] 4. When the reactants meet the required distribution requirements (that is, when the DIFF image is located in the lymphocyte area), add a 1:10 glutaraldehyde-formaldehyde mixture with a final concentration of 0.5% (v / v) in the reaction solution , placed at room temperature for 2 hours.

[0106] 5. Measure on the machine, observe the changes in counting and cell distribution;

[0107] 6. After...

Embodiment 2

[0108] Example 2: Simulating neutrophils with elephant erythrocytes

[0109] 1. Configure the following treatment reagents: add 1.5g sodium chloride, 1g sodium nitrite, 100μl of 1% (w / v) sodium hexadecylsulfonate and 100μl 25% (v / v) pentane to 195ml pure water Dialdehyde;

[0110] 2. Mix 5ml anticoagulant whole blood and processing reagents, and place at room temperature for about 30 minutes;

[0111] 3. Test on the computer, and determine whether to start strengthening the fixation according to different distribution requirements;

[0112] 4. When the reactant meets the required distribution requirements (that is, when the DIFF image is already in the neutrophil area), add 1:10 glutaraldehyde-formaldehyde with a final concentration of 0.5% (v / v) to the reaction solution The mixture was left at room temperature for 2 hours.

[0113] 5. On the machine (BC5500) to measure, observe the changes in count and cell distribution;

[0114] 6. After the requirements are met (that is...

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Abstract

A method for preparing five-part differential leukocyte analogs from red blood cells includes selecting appropriate red blood cells; treating the red blood cells with a multi-functional reagent system for maintenance of a structural integrity of the membranes of the red blood cells and synchronous adjustment of cell volume, morphology and inclusions; subjecting the treated red blood cells to an intensified fixation; and washing the treated cells for preservation. The present disclosure also relates to leukocyte analogs prepared by the above method, a reagent system used for preparing the analogs, and a quality control and a calibrator including the analogs for use in a hematology analyzer.

Description

technical field [0001] The invention relates to the field of blood cell analysis, more specifically, to a five-class leukocyte simulant particle prepared by using red blood cells, a preparation method thereof, and a quality control substance and a calibrator containing the simulant particle. Background technique [0002] In the five-differential hematology analyzer using optical detection technology, the white blood cell particle simulant in its quality control and calibrator is very different from the white blood cell simulant used in the simple impedance method (see US 4,704,364) (see figure 1 ), not only must have a relatively good volume distribution, but also need to have a good cell optical complexity distribution, and some particles also need to have specific fluorescence characteristics. Since the red blood cells of humans or animals are biconcave or spindle-shaped, they cannot show the optical characteristics of concentrated distribution in the optical detection of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/96G01N33/48C12N5/078
CPCC12N5/0641C12N2500/60C12N2503/00
Inventor 张晖王璐徐祖越刘牧龙张丽
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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