Method for extracting lysozyme from egg white

A technology of lysozyme and egg white, applied in the biological field, can solve the problems of low lysozyme efficiency and low lysozyme activity, and achieve the effects of high lysozyme activity and purity, high elution efficiency, and improved extraction efficiency

Active Publication Date: 2009-11-18
浙江艾格生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention provides a method for extracting lysozyme from egg white, which solves the problem that the efficiency of extracting lysozyme by the existing ion exchange adsorption method is not high, and the activity of the extracted lysozyme is not high

Method used

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  • Method for extracting lysozyme from egg white

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Effect test

Embodiment 1

[0030] (1) Take 1000kg of fresh eggs, wash, sterilize and spray, and separate the egg white and egg yolk with an egg beater to obtain 600 liters of egg white.

[0031] (2) Homogenize the egg white with a homogenizer under a pressure of 3Mpa, add water accounting for 15% of the total volume of the egg white to dilute the egg white.

[0032] (3) Use a centrifugal pump to flow the diluted egg white through the Na resin in the adsorption column at a flow rate of 3 L / min.

[0033] (4) After the adsorption is completed, the Na resin in the adsorption column is backwashed with clear water to remove the remaining egg white.

[0034] (5) Then rinse the Na resin in the adsorption column with a 0.5% NaCl solution by weight to remove impurity proteins.

[0035] (6) Finally, use 600L of NaCl solution with a concentration of 2% by weight to elute the Na resin in the adsorption column with a flow rate of 4L / min, collect the eluate, and then continue to increase the concentration of the NaCl...

Embodiment 2

[0039] (1) Take 1000kg of fresh eggs, wash, sterilize and spray, and separate the egg white and egg yolk with an egg beater to obtain 600 liters of egg white.

[0040] (2) Homogenize the egg white with a homogenizer under a pressure of 6Mpa, add water accounting for 5% of the total volume of the egg white to dilute the egg white.

[0041] (3) Use a centrifugal pump to flow the diluted egg white through the Na resin in the adsorption column at a flow rate of 2L / min.

[0042] (4) After the adsorption is completed, use clean water to backwash the Na resin in the adsorption column to remove the remaining egg white.

[0043] (5) Then rinse the Na resin in the adsorption column with a 0.8% NaCl solution by weight to remove impurity proteins.

[0044] (6) Finally, 4% NaCl solution with 600L weight percent concentration is used to elute the Na resin in the adsorption column with a flow rate of 5L / min, collect the eluent, and then continue to increase the concentration of the NaCl sol...

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Abstract

The invention discloses a method for extracting lysozyme from egg white, comprising the following steps: (1) taking the egg white which is added with water to be diluted after being homogenized and flows through an adsorption column filled with Na resin formed by converting from macropore weak acid cation exchange resin; (2) back flushing the Na resin in the adsorption column with water to remove the residual egg white; (3) flushing the Na resin in the adsorption column with NaCl solution to remove foreign protein; (4) carrying out gradient elusion on the Na resin by the NaCl solution and combining all the eluate; (5) separating, condensing and spray drying the eluate via a biomembrane to prepare the finished lysozyme product. The method of the invention adopts the ion exchange resin to adsorb the egg white, enlarges the exchange capacity of the resin, increases the extraction efficiency and ensures relatively high activity and purity of the extracted lysozyme.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting lysozyme from egg white. Background technique [0002] Lysozyme, also known as muramidase or N-acetylmuramide glycanohydrlase, is an alkaline enzyme that can hydrolyze mucopolysaccharides in pathogenic bacteria. Mainly by destroying the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, the insoluble mucopolysaccharides in the cell wall are decomposed into soluble glycopeptides, resulting in the escape of the contents of the cell wall Bacteria dissolve. Lysozyme can also directly combine with negatively charged viral proteins, and form double salts with DNA, RNA, and apoproteins to inactivate the virus. Therefore, the enzyme has antibacterial, anti-inflammatory, antiviral and other effects. [0003] The enzyme is widely present in various tissues of the human body. It is also contained in egg whites of birds and pou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/36
Inventor 徐克成
Owner 浙江艾格生物科技股份有限公司
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