Method for preparing immobilized fructosyltransferase through ion exchange fibers

Abstract

The invention provides a method for preparing immobilized fructosyltransferase through ion exchange fibers. The weakly basic anion exchange fibers are used as a carrier to immobilize a liquid free fructosyltransferase to the carrier, the activity of the immobilized fructosyltransferase can be maintained for a long time, and the immobilized fructosyltransferase can be repeatedly used for a long time. The method comprises two pieces of technological content, namely the liquid free fructosyltransferase is prepared through biolysis and the immobilized fructosyltransferase is prepared through the ion exchange fibers. Fermentation cultivation is conducted on aspergillus oryzae to obtain a large amount of thallus, the thallus is washed, celluloses are used for dissolving the thallus, free enzyme fluid is extracted, and then the fructosyltransferase is immobilized through the ion exchange fibers. The activity of the obtained immobilized fructosyltransferase is larger than or equal to 180 U / g, the half-life period of the immobilized fructosyltransferase is larger than or equal to 40 times, the saccharose inversion rate is larger than or equal to 90%, and the content of fructo-oligose generated through the saccharose inversion is 55-60%. Compared with the public prior art, the method has the advantages that enzyme extraction is simple, operation time is short, enzymes can be easily recycled, the immobilization process is conducted without cross-linking agents, and the product quality and safety are high.

Description

technical field [0001] The invention relates to a method for immobilizing fructosyltransferase, in particular to a preparation method for immobilizing fructosyltransferase by using ion-exchange fibers, which includes fermentation of mycelium with high activity of fructosyltransferase, enzymatic cell decomposition and extraction of enzyme liquid, Immobilization of fructosyltransferase on ion-exchange fibers. Background technique [0002] Ion Exchange Fiber (IEF for short) is a fibrous ion exchange material. As a new functional polymer material, ion exchange fiber has unique chemical and physical adsorption and separation functions, and plays an irreplaceable role in some related fields. . Ion exchange fibers have the following characteristics: various shapes, can be made into long and short fibers, can also be woven and braided, easy to make various components, and can choose the best shape according to the application purpose, large exchange capacity, specific surface ar...

AI Technical Summary

Problems solved by technology

[0004] The above immobilized fructosyltransferases have better industrial application prospects than free enzymes, and realize the characteristics of recyclable and repeated use. However, the above methods are complicated to prepare, the activity after immobilization is not high, and the half-life of the immobilized enzyme is short. , Using more chemical reagents, using toxic glutaraldehyde as a cross-linking agent, due to the enzyme falling off in the middle of the reaction process, the free glutaraldehyde will cause food safety risks to the product, and the mechanical resistance of immobilized enzymes is poor.