High-efficiency liquid phase chromatography detection method for PDE-5 inhibitor in Chinese patent drug, health food and food
A high performance liquid chromatography, health food technology, applied in the field of high performance liquid chromatography detection of PDE5 inhibitors in medicine, health food and food, can solve the high requirements of instrument operators, high instrument price, high maintenance cost problems, to achieve the effect of saving inspection costs, short analysis cycle, and high accuracy
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[0023] In order to make the present invention easier to understand, the present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention, and specific experimental methods are not mentioned in the following examples. Usually carried out according to routine experimental methods.
[0024] The high performance liquid chromatograph of the present invention is mainly equipped with a reversed-phase chromatographic column with octadecylsilane bonded silica gel as a filler, a multi-component pump, a diode array detector, and the like.
[0025] Main analytical steps of the present invention are as follows:
[0026] 1. Chromatographic conditions
[0027] (1) Chromatographic column: a liquid chromatography column using octadecylsilane bonded silica gel as a filler.
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