High-efficiency liquid phase chromatography detection method for PDE-5 inhibitor in Chinese patent drug, health food and food

Abstract

This invention discloses a high-efficiency liquid phase chromatography detection method for PDE-5 inhibitor in Chinese patent drug, health food and food. Diode array detector is adopted, and octadecylsilane chemically bonded silica is used as liquid phase chromatographic column of filling agent; the mobile phase is phosphoric acid triethylamine solution-methanol-acetonitrile system; the flow rate is 0.5 to 1.5 ml/min; the column temperature is 20 to 50 degrees centigrade; the pre-process of the sample uses acetonitrile or methanol for ultrasonic extraction; the sample is analyzed in the wavelength range from 200 mm to 400 mm. This invention has simple pre-process method, high accuracy, good specificity, short analysis cycle and wide detection concentration range; one analysis can detect eleven kinds of PDE-5 inhibitors; the invention is applied to qualitatively and quantitatively analyze PDE-5 inhibitor in Chinese patent drug, health food and food, which can greatly improve detection efficiency, save detection cost and meet the daily monitoring and detecting requirement.

Description

technical field [0001] The invention relates to a high performance liquid chromatography detection method, in particular to a PDE in medicine, health food and food 5 A high-performance liquid chromatography method for the detection of type inhibitors. Background technique [0002] Illegal addition of chemical ingredients in Chinese patent medicines for nourishing kidney and strengthening yang, health food and food is becoming more and more rampant, and there are more and more high-tech counterfeiting activities aimed at standards, which seriously threaten the health of the people. After reviewing a large number of literatures, it was found that the illegally added aphrodisiac chemical ingredients are mainly PDE 5 Type inhibitor (phosphodiesterase type 5 inhibitor, phosphodiesterase type-5 inhibitor) is the main one. The U.S. Food and Drug Administration (FDA) has successively approved three PDEs of sildenafil, vardenafil and tadalafil 5 Type inhibitors, becoming the earli...

AI Technical Summary

Problems solved by technology

In the prior art, the PDE in Chinese patent medicines, health food and food for tonifying kidney and strengthening yang 5 Liquid-mass spectrometry is mainly used for the detection of type inhibitors, but the instrument is too expensive, the maintenance cost is high, and the requirements for instrument operators are also high. It can only be used in a few laboratories with good detection conditions. A Simple, Accurate, and Simultaneous Determination of Multiple Chemical Components by High Performance Liquid Chromatography

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