High-efficiency liquid phase chromatography detection method for PDE-5 inhibitor in Chinese patent drug, health food and food

A high performance liquid chromatography, health food technology, applied in the field of high performance liquid chromatography detection of PDE5 inhibitors in medicine, health food and food, can solve the high requirements of instrument operators, high instrument price, high maintenance cost problems, to achieve the effect of saving inspection costs, short analysis cycle, and high accuracy

CN101587102AActive Publication Date: 2009-11-25广东省药品检验所
0 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2009-11-25

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

This invention discloses a high-efficiency liquid phase chromatography detection method for PDE-5 inhibitor in Chinese patent drug, health food and food. Diode array detector is adopted, and octadecylsilane chemically bonded silica is used as liquid phase chromatographic column of filling agent; the mobile phase is phosphoric acid triethylamine solution-methanol-acetonitrile system; the flow rate is 0.5 to 1.5 ml / min; the column temperature is 20 to 50 degrees centigrade; the pre-process of the sample uses acetonitrile or methanol for ultrasonic extraction; the sample is analyzed in the wavelength range from 200 mm to 400 mm. This invention has simple pre-process method, high accuracy, good specificity, short analysis cycle and wide detection concentration range; one analysis can detect eleven kinds of PDE-5 inhibitors; the invention is applied to qualitatively and quantitatively analyze PDE-5 inhibitor in Chinese patent drug, health food and food, which can greatly improve detection efficiency, save detection cost and meet the daily monitoring and detecting requirement.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a high performance liquid chromatography detection method, in particular to a PDE in medicine, health food and food 5 A high-performance liquid chromatography method for the detection of type inhibitors. Background technique

[0002] Illegal addition of chemical ingredients in Chinese patent medicines for nourishing kidney and strengthening yang, health food and food is becoming more and more rampant, and there are more and more high-tech counterfeiting activities aimed at standards, which seriously threaten the health of the people. After reviewing a large number of literatures, it was found that the illegally added aphrodisiac chemical ingredients are mainly PDE 5 Type inhibitor (phosphodiesterase type 5 inhibitor, phosphodiesterase type-5 inhibitor) is the main one. The U.S. Food and Drug Administration (FDA) has successively approved three PDEs of sildenafil, vardenafil and tadalafil 5 Type inhibitors, becoming the earli...

Examples

Embodiment Construction

[0023] In order to make the present invention easier to understand, the present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention, and specific experimental methods are not mentioned in the following examples. Usually carried out according to routine experimental methods.

[0024] The high performance liquid chromatograph of the present invention is mainly equipped with a reversed-phase chromatographic column with octadecylsilane bonded silica gel as a filler, a multi-component pump, a diode array detector, and the like.

[0025] Main analytical steps of the present invention are as follows:

[0026] 1. Chromatographic conditions

[0027] (1) Chromatographic column: a liquid chromatography column using octadecylsilane bonded silica gel as a filler.

[0028] (2) Detector: diode array dete...