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Method for fast analyzing pesticide residue in fruits and vegetables

A technology with many pesticide residues and rapid analysis, applied in the field of food testing, can solve the problems of low sample preparation efficiency, restricting detection efficiency, time-consuming and labor-intensive problems, and achieve the effect of simple operation, less solvent consumption, and avoiding losses

Active Publication Date: 2011-11-09
烟台杰科检测服务有限公司
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Problems solved by technology

[0003] In recent years, the development of testing instruments has been changing with each passing day. However, while the detection efficiency of the instrument has been improved, the traditional sample pretreatment technology has greatly restricted the further improvement of the detection efficiency. For the detection of pesticide residues in fruits and vegetables, the current multi-residue analysis method There are GB / T 19648-2006, GB / T20769-2006 and Japan's Qi analysis method, etc. These methods all use traditional ultrasonic or homogenization for extraction, and the amount of reagents used is relatively large, plus solid phase extraction SPE column is used for enrichment, concentration, and purification. The sample preparation efficiency is low and the cost is relatively high, and it is time-consuming and laborious. An efficient pretreatment method is urgently needed to replace it. Residue analysis methods are particularly important

Method used

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  • Method for fast analyzing pesticide residue in fruits and vegetables
  • Method for fast analyzing pesticide residue in fruits and vegetables
  • Method for fast analyzing pesticide residue in fruits and vegetables

Examples

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Embodiment 1

[0026] Example 1, a rapid analysis method for pesticide residues in fruits and vegetables, taking green asparagus as an example, preparing 130 kinds of pesticide mixed standard solutions, adding 0.01mg / kg and 0.1mg / kg pesticide standard substances for recovery, each Each content level was repeated 4 times, then extracted, and 15.0 g of uniformly prepared samples were weighed, added to a 50 mL centrifuge tube containing 1.5 g of anhydrous sodium acetate and 6 g of anhydrous magnesium sulfate, and then added with 15 mL of 1% acetic acid acetonitrile solution, vigorously Shake for 1min, after ultrasonic extraction for 15min, centrifuge at 5000r / min for 1min; purify, transfer 10mL of acetonitrile extract to a 15mL centrifuge tube filled with mixed fillers in different proportions, the filler is 500mg PSA and graphitized carbon in a mass ratio of 1:1, Shake for 1min, centrifuge at 5000r / min for 1min, absorb 5mL of purified solution, concentrate and evaporate to dryness, and make up ...

Embodiment 2

[0038] Example 2, a rapid analysis method for multiple residues of pesticides in fruits and vegetables, taking edamame as an example, preparing 28 kinds of pesticide mixed standard solutions, adding and recovering at a level of 0.02 mg / kg, repeating 4 times for each content level, and then extracting , Weigh 15.0g of the uniformly prepared sample, add it to a 50mL centrifuge tube containing 1.5g of anhydrous sodium acetate and 6g of anhydrous magnesium sulfate, then add 15mL of 1% acetic acid acetonitrile solution, shake vigorously for 1min, and after ultrasonic extraction for 15min, 5000r / min centrifugation for 1min; purification, transfer 10mL of acetonitrile extract to a 15mL centrifuge tube filled with different proportions of mixed fillers, the fillers are 500mg graphitized carbon, PSA, C 18 The mass ratio of the mixture is 1:2:2, shaken for 1min, centrifuged at 5000r / min for 1min, absorbs 5mL of purified solution, concentrates and evaporates to dryness, and the volume ra...

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Abstract

The invention discloses a method for fast analyzing pesticide residue in fruits and vegetables, which is implemented by weighing a sample, adding anhydrous sodium acetate and anhydrous magnesium sulfate, adding acetic acid acetonitrile solution, centrifugating after shaking and extracting, putting the centrifugated acetonitrile extract into a centrifugating tube filled with mixed filler, shaking,centrifugating, sucking the cleaning fluid, concentrating and evaporating to be dry, dissolving the mixed solution with the volume ratio of acetonitrile and water being 1:1, and performing UPLC / MS / MSdetection. The invention uses fruits and vegetables as matrix, uses the matrix dispersion solid extraction technology to pre-treat the sample to realize fast detection on 130 types of pesticide residue such as avermectins, aldicarb, Cyromazine and the like, having the characteristics of fast and exact detection result, high efficiency and low price.

Description

technical field [0001] The invention belongs to the technical field of food detection methods, and in particular relates to a rapid analysis method for multiple residues of pesticides in fruits and vegetables. Background technique [0002] As countries strengthen the safety inspection of imported food, such as the implementation of Japan's positive list, the limit standards of various agricultural products are getting lower and lower, and the scope of pesticides is getting wider and wider, and the export situation has become very severe. While food safety is getting more and more attention, all countries are trying to find a simple, fast, accurate, efficient, and cheap multi-residue analysis method to meet the needs of food safety in the future. [0003] In recent years, the development of testing instruments has been changing with each passing day. However, while the detection efficiency of the instrument has been improved, the traditional sample pretreatment technology has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 娄喜山付建高洪良
Owner 烟台杰科检测服务有限公司
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