Mutant of trehalose synthetase from corynebacterium glutamicum and application thereof

Abstract

The invention relates to a mutant of trehalose synthetase from corynebacterium glutamicum. A method for obtaining the mutant performs site-directed saturation mutation on a phenylalanine residue at the 263rd site of an amino acid sequence of trehalose synthetase from corynebacterium glutamicum by using a site-directed mutation technology, and the method for obtaining the mutant also comprises the following step: the saturation mutation is performed on at least one amino acid residue in at least 80 percent of homologous trehalose synthetase sequences of an amino acid sequence including SEQ ID NO.1, SEQID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The reaction kinetics of the trehalose synthetase expressed by a mutated trehalose synthetase gene changes, various enzymology characteristics such as specific activity of enzyme, optimum reaction temperature, optimum pH value, and the like also change, and the enzyme can improve the transformation efficiency of the trehalose synthetase and further reduce the production cost of the trehalose.

Description

technical field

[0001] The invention relates to the technical field of modern enzyme engineering, in particular to a mutant of Corynebacterium glutamicum trehalose synthase and application thereof. Background technique

[0002] Trehalose, more precisely α,α-1,1-trehalose is composed of two molecules of glucose connected by α,α-1,1-glycosidic bonds. Trehalose is a natural disaccharide widely present in microorganisms, shrimps, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. But the content is very small. In the past, it was mainly extracted from dry yeast. Due to the low content, the extraction process is complicated and the cost is extremely high. Such expensive trehalose can only be limited to some special fields such as the application of activity maintenance of medical biological products. With the development and progress of human society, it is becoming more and more obvious to apply trehalose to maintain the umami taste and improve the ...

Images