Microbial preparation for preventing and treating mastitis and applications thereof

A technology of microbial preparations and mastitis, applied in the field of microbial preparations, can solve problems such as low production efficiency, incompatibility, and labor consumption, and achieve the effects of wide application range, significant effect, and overcoming side effects

Inactive Publication Date: 2010-02-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these treatments are time-consuming, labor-intensive, and require individual handling of

Method used

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  • Microbial preparation for preventing and treating mastitis and applications thereof
  • Microbial preparation for preventing and treating mastitis and applications thereof
  • Microbial preparation for preventing and treating mastitis and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of microbial preparations for preventing and treating mastitis

[0039] The Bdellovibrio strains used were Bdellovibrio BDS01 and Bdellovibrio BDS02, respectively. Bdellovibrio BDS01 was preserved in the China Center for Type Culture Collection on August 7, 2009, and the preservation number is CCTCC NO: M 209169; after negative staining of the Bdellovibrio BDS01, the morphology was observed under an electron microscope: BDS01 showed a single The cells are arc-shaped, with a size of 0.9×0.25 μm, terminal flagella, and the length of the flagella is 4 μm. They can form transparent circular plaques with a diameter of 1-2 mm when cultured at 28° C. for four days by double-layer plate method. The Bdellovibrio BDS02 was preserved in the China Center for Type Culture Collection on August 7, 2009, and the preservation number is CCTCCNO: M 209170; after the Bdellovibrio BDS02 was negatively stained, the morphology was observed under an electron microscope: BDS02 w...

Embodiment 2

[0063] Microbial preparations are used to treat small-tailed cold sheep infected with mastitis, and the specific process is as follows:

[0064] A, test drug: experimental group: the microbial preparation that embodiment 1 step (1) prepares

[0065] Control group: ciprofloxacin lactate (produced by Luoyang Huizhong Veterinary Medicine Co., Ltd.);

[0066] B. Treated cases: A total of 120 cases of clinical mastitis were treated, including 20 cases in the ciprofloxacin lactate group and microbial preparations A, B, C, D, and E groups;

[0067] C. Dosage and method: The dosage is as follows: ciprofloxacin lactate 1.5% 10mL; Bdellovibrio 103pfu / ml 1000mL; the medication method is as follows: ① Squeeze the milk first, disinfect the nipple with 75% alcohol by volume, and insert the milk duct For the breast, inject ciprofloxacin lactate, then grasp the nipple and massage the breast sequentially from bottom to top, so that the liquid medicine spreads throughout the breast, 2 times a ...

Embodiment 3

[0073] (1) Preparation of microbial preparations for preventing and treating mastitis in sows

[0074] The preparation process is the same as Example 1 step (1), and the difference is only that the solution of suspended hiruplasma and swimming body used when preparing microbial preparations A, B, C, D, E uses PBS damping fluid (0.2mol / L, pH is 7.2).

[0075] (2) Treatment of pigs infected with mastitis with microbial preparations

[0076] A, test drug: experimental group: microbial preparation prepared in step (1)

[0077] Control group: 0.5-1% procaine hydrochloride, penicillin (produced by Sichuan Dikang Technology Pharmaceutical Co., Ltd.);

[0078] B. Treated cases: A total of 120 cases of clinical mastitis were treated, including 20 cases in the ciprofloxacin lactate group and microbial preparations A, B, C, D, and E groups;

[0079] C. Dosage and method: The dosage is as follows: procaine hydrochloride 0.5-1% 15mL, penicillin 400,000 international units; Bdellovibrio ...

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Abstract

The invention discloses a microbial preparation for preventing and treating mastitis and applications thereof. Bdellovibrio telotroch and Bdellovibrio plastid are prepared into the microbial preparation for preventing and treating mastitis. By detection, the microbial preparation has favorable functions for preventing and treating mastitis of pigs, sheep and cows. The microbial preparation using the mixture of the Bdellovibrio telotroch and the Bdellovibrio plastid for controlling and eliminating mastitis pathogen has the advantages of quick effect taking and strong activity of the telotroch and the advantages of easy preparation and storage, long sterilization action time and strong environmental resistance. Since Bdellovibrio does not have toxic or side effect on cows, the microbial preparation prepared from the Bdellovibrio telotroch and the Bdellovibrio plastid is suitable for large-size factory culture of pigs, sheep and cows, and can provide a novel method for green processing and production of meat products and milk products and for preventing and treating mastitis pathogen.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a microbial preparation for preventing and treating mastitis and its application. Background technique [0002] Mastitis (also called mastitis) is a common and complex disease that causes serious economic losses in dairy cattle, sheep, and pig farming. The survey results in Beijing, Shanghai and other places in my country show that the incidence of recessive mastitis in dairy cows is about 60%. At present, there are about 220 million dairy cows in the world, about 1 / 3 of which suffer from various types of mastitis, and the annual loss due to mastitis is as high as 35 billion US dollars. Although the disease has been studied for more than 100 years, a thorough solution has not been proposed so far. [0003] Pathogen infection is the main cause of mastitis, including bacteria, fungi, viruses, mycoplasma, etc. The main pathogenic bacteria are bacteria, the common ones are Staphylococcus...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P15/14C12N1/20C12R1/63
Inventor 蔡俊鹏孙丽滢陈小红
Owner SOUTH CHINA UNIV OF TECH
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