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Discrimination method of traditional Chinese medicine Xuezhining pill

A technology of Xuezhining and traditional Chinese medicine, applied in the identification field of traditional Chinese medicine Xuezhining pills, can solve the problems of unfavorable Xuezhining pills quality control, long quality control method, cumbersome identification method steps and the like, achieves quality control method optimization, easy to observe and identify , the effect of speeding up the identification speed

Active Publication Date: 2012-05-23
津药达仁堂集团股份有限公司达仁堂制药厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method is the existing technology for identifying Xuezhining Pills. After careful study of the identification method, it is believed that there are the following problems: the prescription of this Xuezhining Pills is cassia seed, hawthorn, lotus leaf, and Radix Polygoni Multiflori. Sieve, mix well, add 70-90g of refined honey per 100g of powder to make large honeyed pills, namely Xuezhining pills; Polygonum multiflorum and Cassia seeds in this prescription contain anthraquinones, which interferes in identification, which is not conducive to Xuezhining The quality control of pills, and the identification method steps in the prior art are loaded down with trivial details; Have the content determination item in the quality control method of former traditional Chinese medicine Xuezhining pills to be reference substance with ursolic acid, adopt TLC scanning method to measure hawthorn content and existing In the identification method in the technology, Polygonum multiflorum is used as the reference medicinal material and ursolic acid is used as the reference substance. The steps of making Polygonum multiflorum and Hawthorn in the identification prescription are repeated, resulting in long time for the quality control method and low work efficiency.

Method used

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  • Discrimination method of traditional Chinese medicine Xuezhining pill
  • Discrimination method of traditional Chinese medicine Xuezhining pill
  • Discrimination method of traditional Chinese medicine Xuezhining pill

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for identifying the traditional Chinese medicine Xuezhining Pills, which uses thin-layer chromatography to identify anthraquinone active ingredients in Xuezhining Pills and Nuciferine in Xuezhining Pills. The identification of anthraquinone active ingredients in Xuezhining Pills includes the following step:

[0037] (1) Preparation of the test solution: take 5g of the traditional Chinese medicine Xuezhining Pills, cut into pieces, add 20ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 5ml of water to the residue to dissolve, add 0.5ml of hydrochloric acid, and heat to reflux After 30 minutes, let it cool, shake and extract twice with diethyl ether, 10ml each time, combine the diethyl ether solution, evaporate to dryness, add 0.5ml of chloroform to the residue to dissolve, and obtain the test solution;

[0038] (2) Preparation of reference substance solution: take chrysophanol and chrysophanin reference substanc...

Embodiment 2

[0046] A method for identifying the traditional Chinese medicine Xuezhining Pills, which uses thin-layer chromatography to identify the anthraquinone active ingredients in the Xuezhining Pills, including the following steps:

[0047] (1) Preparation of the test solution: take 10g of the traditional Chinese medicine Xuezhining pills, cut them into pieces, add 50ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 30ml of water to the residue to dissolve, add 5ml of hydrochloric acid, and heat to reflux for 30 minutes Minutes, let cool, shake and extract 2 times with diethyl ether, 30ml each time, combine the diethyl ether solution, evaporate to dryness, add 5ml of chloroform to the residue to dissolve, and obtain the test solution;

[0048] (2) Preparation of reference substance solution: get chrysophanol, emodin methyl ether reference substance, add chloroform to make a mixed solution containing 5 mg chrysophanol and emodin methyl ...

Embodiment 3

[0051] A kind of identification method of traditional Chinese medicine Xuezhining Pills, its identification adopts thin-layer chromatography to comprise the following steps in the Nuciferine in Xuezhining Pills:

[0052] (1) Preparation of the test solution: take 20 g of the traditional Chinese medicine Xuezhining pills, cut them into small pieces, add 35 ml of methanol, ultrasonically treat them for 30 minutes, filter, evaporate the filtrate to dryness, add 20 ml of ammonia test solution to the residue to dissolve, and vibrate with chloroform Shake and extract 3 times, 20ml each time, combine the chloroform solution, evaporate to dryness, dissolve the residue with 15ml of 0.01mol / L hydrochloric acid solution, add 20ml chloroform and shake for extraction, discard the chloroform solution, and use the acid water solution Adjust the pH value of the concentrated ammonia solution to 9.5, then shake and extract twice with chloroform, 20ml each time, combine the chloroform solution, e...

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Abstract

The invention relates to a discrimination method of traditional Chinese medicine Xuezhining pill; wherein discrimination of anthraquinones active ingredients in the Xuezhining pill and / or discrimination of nunciferine in Xuezhining pill all adopt thin-layer chromatography. Chrysophanol and physcion thin-layer chromatography in the discrimination method adopts acid hydrolysis method, thus greatly improving thin-layer spot detection effect, being convenient for observation and discrimination and improving discrimination efficiency; lotus leaf thin-layer chromatography in the discrimination method adopts alkalinity developing solvent cyclohexane-ethyl acetate-concentrated ammonium liquid, developing speed can be effectively improved, and discrimination speed of the whole Xuezhining pill can be improved. Repetitive operation of hawthorn thin-layer discrimination method is cancelled in the discrimination method, so that the whole quality control method is optimized, quality control time isshortened, and quality control efficiency is improved. The discrimination method of the invention is simple and easy to operate, eliminates the interference in discrimination as prepared polygonum multiflorum and semen cassiae all contain anthraquinones and is rapid and accurate for discrimination of traditional Chinese medicine Xuezhining pill.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, in particular to a method for identifying traditional Chinese medicine Xuezhining pills. Background technique [0002] Xuezhining Pill is a traditional Chinese medicine used for hyperlipidemia of phlegm-turbidity stagnation type, reducing turbidity and lowering fat, and laxative. This kind of traditional Chinese medicine is developed by extensively consulting relevant medical and pharmaceutical literature such as "Synopsis of the Golden Chamber" and "Treatise on Febrile Diseases", exploring drugs for reducing blood lipids, collecting ancient folk prescriptions and proven prescriptions, and repeatedly screening drugs for lowering blood lipids and blood pressure. , starting from the 1990 edition of a "Chinese Pharmacopoeia", all previous editions of the Pharmacopoeia have been included. Due to the complex composition of Xuezhining Pills, it is very necessary for quality control. Curre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/734A61P3/06G01N30/90
Inventor 王志强李艳钰金兆祥刘淑闫晓楠
Owner 津药达仁堂集团股份有限公司达仁堂制药厂
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