Yeast preserving solution and using method thereof

Abstract

The invention provides yeast preserving solution and also provides a method for preserving yeasts by using the yeast preserving solution and a fermentation composition containing the yeasts and the yeast preserving solution. The yeast preserving solution contains buffer solution serving as a solvent and mycose and adenine which are dissolved in the solvent and can preserve a bacterial strain for 1 to 2 years under a condition of 4 to 10 DEG C or for 1 to 3 weeks at normal temperature. The fermentation composition can be used as a seed to be directly inoculated in a K tank for production without resurrection propagation in a liquid culture medium in a triangular flask, thereby reducing half of the time of a seed production period of a production line.

Description

technical field [0001] The invention relates to a solution for preserving strains and an application method thereof, more specifically, to a solution for preserving yeast and an application method thereof. Background technique [0002] Yeast is the earliest and most widely used microorganism used by humans. People often use its fermentation to make various dough foods and wine. [0003] The development of yeast research and its industrial application is bound to be related to safe and long-term strain preservation technology. For yeast with important economic value, if the physiological and genetic characteristics of the strain are damaged during the preservation process, Mutation will bring huge economic losses. In the industrial production that requires yeast, it is often necessary to use the same batch of yeast strains on the production line stably for a long time (more than 1 year), and sometimes it is necessary to transport the yeast strains over long distances at room...

AI Technical Summary

Problems solved by technology

In the prior art, the following two types of methods are mainly used for the preservation of yeast strains. The first type is the preservation method of allowing the strains to grow continuously, that is, the regular transplantation preservation method, which mainly includes inclined plane transplantation, oil pipe preservation and distilled water preservation, etc. Although this This type of method has the advantages of being easy to operate and not requiring special equipment, but it also has the disadvantages of costing manpower and time, contamination risk, and easy mutation of bacterial strains, and is not suitable for industrial production that requires long-term use of the same batch of bacterial strains; the second The second method is to use the method of inhibiting the metabolic activity of bacteria to preserve, mainly including freeze-drying, low-temperature freezing and liquid nitrogen preservation. These methods can preserve bacteria for a long time, and the bacteria are not easy to mutate, but the methods are generally complicated and Need to use expensive equipment, the cost is high; therefore, there is a need for a low-cost and long-term stable preservation method of yeast strains even at room temperature for a certain period of time, especially the method of preserving yeast strains in liquid form

[0004] In the prior art, there are methods for preserving strains in liquid form. For example, WO0039281A2 discloses a liquid culture of microbial strains (including yeast), which contains compounds related to nucleic acid biosynthesis ( Including adenine) and optionally added trehalose, but the storage temperature of the bacteria in this method is low, which is -20°C to 0°C, which is not suitable for storage at room temperature, so it is not convenient for long-distance transportation. In addition, this method The storage time of the strains is short, no more than 16 weeks, so it is not suitable for long-term and stable use of the same batch of strains on the production line for more than 1 year

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