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Analytical method for detecting trace mercury in aqueous medium based on the color change of fluorescence

A technology for detecting water and medium, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of few reports of direct sensitive detection, and achieve the effect of high sensitivity, convenient detection and high repeatability

Inactive Publication Date: 2011-04-06
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, using Hg based on water-soluble co-polymers, especially water-soluble co-oligomers 2+ Ion fluorescence sensors are rarely reported
Moreover, based on the change of fluorescent color that can be recognized by naked eyes, the effect on Hg 2+ There are fewer reports on the direct and sensitive detection of ions

Method used

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  • Analytical method for detecting trace mercury in aqueous medium based on the color change of fluorescence
  • Analytical method for detecting trace mercury in aqueous medium based on the color change of fluorescence
  • Analytical method for detecting trace mercury in aqueous medium based on the color change of fluorescence

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Experimental program
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Effect test

Embodiment 1

[0031] Weigh an appropriate amount of the synthesized conjugated oligopyrene derivatives and the homopolynucleic acid containing 8 bases T into two reagent bottles, and dissolve them with ultrapure water to prepare an aqueous solution with an equimolar concentration of 2.5 ×10 -5 mol L -1 . The two solutions were then mixed at room temperature and allowed to stand for 10 min. Pipette 13 portions of 5 mL of this solution as a test solution. Then, drop the K to be measured into the system + , Ba 2+ , Ca 2+ , Cd 2+ ,Co 2+ , Cu 2+ , Fe 3+ , Fe 2+ , Mg 2+ , Mn 2+ , Ni 2+ , Pb 2+ , Zn 2+ sample. The final concentration of each ion is 1.25×10 -4 mol L -1 mol L -1 . Complex detection solution for Hg 2+ The fluorescent response of the fluorescent sensor system was excited by a portable UV-ultraviolet lamp at 25°C, and the excitation wavelength was 365nm. The results showed that the blue fluorescence of the sensing system was not affected by the relevant alkali me...

Embodiment 2

[0033] Weigh an appropriate amount of the synthesized conjugated oligopyrene derivative and the homopolynucleic acid containing 8 bases T into two reagent bottles respectively, and use the concentration of 10mmol L -1 ; The Tris-HCl buffer solution of pH=7.5 is dissolved, and is prepared into an aqueous solution of equimolar concentration, and its concentration value is 2.5×10 -5 mol L -1 . The two solutions were then mixed at room temperature and allowed to stand for 10 min. Pipette 13 portions of 5 mL of this solution as a test solution. Then, drop the K to be measured into the system + , Ba 2+ , Ca 2+ , Cd 2+ ,Co 2+ , Cu 2+ , Fe 3+ , Fe 2+ , Mg 2+ , Mn 2+ , Ni 2+ , Pb 2+ , Zn 2+ sample. The final concentration of each ion is 1.25×10 -4 moL -1 mol L -1 . Complex detection solution for Hg 2+ The fluorescent response of the fluorescent sensor system was excited by a portable UV-ultraviolet lamp at 25°C, and the excitation wavelength was 365nm. The result...

Embodiment 3

[0035] Weigh an appropriate amount of the synthesized conjugated oligopyrene derivative and the homopolynucleic acid containing 8 bases T into two reagent bottles respectively, and use the concentration of 10mmol L -1 ; The Tris-HCl buffer solution of pH=7.5 is dissolved, and is prepared into an aqueous solution of equimolar concentration, and its concentration value is 2.5×10 -5 mol L -1 . Then the two solutions were mixed at 40°C and allowed to stand for 10 min. Pipette 13 portions of 5 mL of this solution as a test solution. Then, drop the K to be measured into the system + , Ba 2+ , Ca 2+ , Cd 2+ ,Co 2+ , Cu 2+ , Fe 3+ , Fe 2+ , Mg 2+ , Mn 2+ , Ni 2+ , Pb 2+ , Zn 2+ sample. The final concentration of each ion is 1.25×10 -4 mol L -1 . Complex detection solution for Hg 2+ The fluorescent response of the fluorescent sensor system was excited by a portable UV-ultraviolet lamp at 25°C, and the excitation wavelength was 365nm. The results showed that the blu...

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Abstract

The invention relates to a highly sensitive and selective identification method for detecting Hg<2+> in aqueous medium based on the color change of fluorescence in the sensor system. The method comprises the following specific steps: (1) synthesizing water-soluble pyrene oligomer derivative through chemical and electrochemical technologies; and (2) performing molecular combination in aqueous phase between the derivative and short chain homopolynucleic acid with rich base T in the molar ratio of 1:1 and obtaining fluorescence probes used for the Hg<2+> detection. The invention adopts the colorchange of fluorescence in the sensor system caused by the pi electron interaction between molecular chains induced by Hg<2+> so as to realize the highly sensitive and selective detection of trace Hg<2+> in aqueous medium, provide an effective, simple and intuitive analysis method of the detection of trace ions in aqueous medium and have important meaning and actual application value for the detection of Hg<2+> in environmental water.

Description

technical field [0001] The invention relates to an analysis method for detecting trace mercury in water medium based on fluorescence color change, and belongs to the technical field of material preparation. technical background [0002] The pollution of heavy metal ions can damage the ecological environment and endanger human health. Among them, the pollution of mercury ions is particularly valued, because its pollution is ubiquitous, and low concentrations of mercury can accumulate in organisms, causing disorders of human cognitive ability and motor function, and damaging liver and kidney functions. , Break the balance between the immune system. Therefore, the design and synthesis of highly sensitive fluorescent sensors for mercury ion detection has become one of the hotspots in the field of scientific research today. However, a great challenge is to develop a method that can detect Hg in water 2+ Inexpensive, sensitive sensor with high selectivity to distinguish interfe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 陈友强张新霓
Owner QINGDAO UNIV
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