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Method for establishing atherosclerosis cell model

A technology of atherosclerosis and cell models, applied in biochemical equipment and methods, organic chemistry, steroids, etc., can solve the problems of time-consuming animal models, unreported atherosclerosis cell models, and cost-consuming problems

Active Publication Date: 2010-04-07
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chen Jie et al. [Anatomy Research, 2007, Volume 29 No. 03] reported the establishment of a mouse model of atherosclerosis, but the establishment of animal models is time-consuming and expensive
There is currently no report on the use of oxLig-1 to establish a cell model of atherosclerosis

Method used

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  • Method for establishing atherosclerosis cell model
  • Method for establishing atherosclerosis cell model
  • Method for establishing atherosclerosis cell model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Chemical synthesis and structural analysis of oxLig-1:

[0044] 1. Synthesis of oxLig-1:

[0045] (1) Create a response:

[0046] 0.500mmol of azelaic acid, 0.1875mmol of DCC and 0.125mmol of catalyst DMAP were dissolved in 15mL of dichloromethane, and 0.125mmol of 7-KC, which was also dissolved in dichloromethane, was added dropwise from a constant pressure funnel, and the molar ratio of each substance was azelaic Acid: DCC: DMAP: 7-KC = 4: 1.5: 1: 1.

[0047]

[0048] The reactant was stirred and reacted at 40° C. for 12 hours, and then refluxed for 12 hours, during which time the reaction was monitored by thin-layer chromatography.

[0049] (2) post-reaction treatment:

[0050] The reaction product was filtered under reduced pressure to remove insoluble urea; extracted 3 times with saturated NaCl, and the organic phase was collected; anhydrous NaCl 2 SO 4 Let dry overnight.

[0051] (3) Sample mixing:

[0052] Weigh the mixed sample silica gel (100-140 m...

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Abstract

The invention relates to a method for establishing an atherosclerosis cell model. The method comprises the following steps: using an improved method for synthesizing an oxLig-1; using the oxLig-1 as an irritant which is used for promoting the activation of a signal channel of PKC and NF-kappaB; using the oxLig-1 energy to induce a mononuclear cell (THP-1) to be differentiated into macrophages; after the oxLig-1 acts on J774A.1 cells for 12 hours, accumulating fats in the macrophages to form foam cells to finally cause apoptosis. The method has the following advantages of replacing a time-consuming animal model by establishing the atherosclerosis cell model, and providing an effective means for the mechanism research of atherosclerosis.

Description

technical field [0001] The invention relates to a method for establishing an atherosclerosis cell model. Background technique [0002] It has been known that many components in oxLDL play a vital role in the formation of atherosclerotic plaque and the occurrence and development of atherosclerosis. The study also found that β 2 -The specific ligand of GPI, oxLig-1, has strong immune activity and plays a crucial role in the development of atherosclerosis (Liu et al., J Lipid Res 2002, 43:1486- 95). PCT / JP02 / 00723 discloses the method of chemically synthesizing oxLig-1 by using WSC / DMAP catalyst system to synthesize oxLig-1. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (WSC) and 4-dimethylaminopyridine (DMAP) were used as catalysts for the synthesis reaction. However, the disadvantage of this method is that the amount of oxLig-1 synthesis is not high, and the reaction time is longer, which takes 2-3 days. [0003] In the early stage of atherosclerosis, NF-κB...

Claims

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Application Information

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IPC IPC(8): C12N5/08C07J9/00
Inventor 刘庆平李文哲黄振宇
Owner DALIAN UNIV