Preparation of anti-coagulation dermis scaffold

A technology of dermis and preparation steps, applied in the field of medical devices, can solve problems such as unfavorable capillaries, coagulation contact, ingrowth, etc., and achieve the effects of reducing coagulation, maintaining activity, and simple preparation method

Active Publication Date: 2014-12-24
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the case of a large area of ​​skin trauma, when it needs to be repaired with the assistance of artificial skin, if a large amount of blood coagulation occurs, scars appear, and the dermis of the artificial skin is in contact with necrotic tissue, it is not conducive to the growth of capillaries into the scaffold.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The ratio of silk fibroin and chitosan was 1:3, and the silk fibroin / chitosan / heparin artificial dermal scaffold containing 0.01% heparin was prepared as follows:

[0039] 1. Prepare a 0.2M solution of acetic acid. Dissolve 1.2 g of acetic acid in deionized water and titrate to 100 mL to obtain a 0.2 M acetic acid solution.

[0040] 2. Prepare the heparin solution. Dissolve 0.16 mg of heparin in 40 mL of the acetic acid solution prepared in step 1, and then titrate with acetic acid to the pH value of the solution before adding heparin.

[0041] 3. Prepare a 3% chitosan / heparin solution. Take 40mL of the heparin solution prepared in step 2 and dissolve 1.2g of chitosan to obtain a milky white viscous liquid.

[0042] 4. Prepare silk fibroin solution. Make the concentration of silk fibroin in the solution be 4%.

[0043] 5. Prepare silk fibroin / chitosan / heparin mixed solution. Add the 40mL acetic acid / heparin solution prepared in step 3 into the 9mL silk fibroin / chi...

Embodiment 2

[0048] The ratio of silk fibroin and chitosan was 3:1, and the silk fibroin / chitosan / heparin artificial dermal scaffold containing 0.04% heparin was prepared as follows:

[0049] 1. Prepare a 0.2M solution of acetic acid. Dissolve 1.2 g of acetic acid in deionized water and titrate to 100 mL to obtain a 0.2 M acetic acid solution.

[0050] 2. Prepare the heparin solution. Dissolve 2 mg of heparin in 40 mL of the acetic acid solution prepared in step 1, and then titrate with acetic acid to the pH value of the solution before adding heparin.

[0051] 3. Prepare a 3% chitosan / heparin solution. Take 40mL of the heparin solution prepared in step 2 and dissolve 1.2g of chitosan to obtain a milky white viscous liquid.

[0052] 4. Prepare silk fibroin solution. Make the concentration of silk fibroin in the solution be 4%.

[0053] 5. Prepare silk fibroin / chitosan / heparin mixed solution. Add the 40ml chitosan / heparin solution prepared in step 3 to the 80mL silk fibroin solution pre...

Embodiment 3

[0058] The ratio of silk fibroin and chitosan is 1:1, and the silk fibroin / chitosan / heparin artificial dermal scaffold containing 0.1% heparin is prepared as follows:

[0059] 1. Prepare a 0.2M solution of acetic acid. Dissolve 1.2 g of acetic acid in deionized water and titrate to 100 mL to obtain a 0.2 M acetic acid solution.

[0060] 2. Prepare the heparin solution. Dissolve 2.4 mg of heparin in 40 mL of the acetic acid solution prepared in step 1, and titrate with acetic acid to the pH value of the solution before adding heparin.

[0061] 3. Prepare a 3% chitosan / heparin solution. Take 40mL of the heparin solution prepared in step 2 and dissolve 1.2g of chitosan to obtain a milky white viscous liquid.

[0062] 4. Prepare silk fibroin solution. Make the concentration of silk fibroin in the solution be 4%.

[0063] 5. Prepare silk fibroin / chitosan / heparin mixed solution. Add the chitosan / heparin solution prepared in step 3 to 30mL of the silk fibroin solution prepared ...

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Abstract

The invention relates to a preparation of anti-coagulation dermis scaffold which is used for skin injury repair and has good three-dimensional porous structure. The scaffold preparation uses three natural biologicals of regeneration silk fibroin, chitosan and heparin as raw materials, wherein, heparin has outstanding anti-coagulation property. The dermis scaffold has high porosity and aperture size appropriate for dermal cells to grow; the scaffold has better mechanical property and good hydrophily; the artificial dermis scaffold has heparin slow release function, so that the protrombin time, partial thromboplastin time and thrombin time thereof can be obviously prolonged, thus proving that the dermis scaffold has excellent anti-coagulation property.

Description

technical field [0001] The invention belongs to the field of medical equipment. More precisely, the present invention relates to a method for preparing an artificial dermal scaffold for repairing skin injuries, having good three-dimensional porous structure and anticoagulant property. technical background [0002] The skin is the largest organ of the human body and has the functions of retaining moisture in the body, preventing excessive heat dissipation, ventilating and preventing bacterial invasion. When the human body is damaged by trauma, burns, inflammation, etc., especially when a large area of ​​skin is severely burned or damaged, the wound should be protected immediately. If only a superficial layer or a small area of ​​skin is damaged, new skin will regenerate itself. However, if there is a severe trauma, the skin cannot repair itself. Usually, the superficial skin of other parts of the body must be grafted onto the wound. [0003] Since there is no alternative so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/54A61L27/56A61L27/60A61L33/10
Inventor 佘振定王明波谭荣伟
Owner SHENZHEN QIKANG MEDICAL DEVICES
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