Integrated peptidoglycan and preparation method thereof
A peptidoglycan and a complete technology, applied in the field of pharmaceutical preparations, can solve problems such as lack of Lactobacillus bulgaricus extraction, and achieve the effects of suitable large-scale production, anti-tumor effect and good effect
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Embodiment 1
[0029] The teichoic acid of Lactobacillus bulgaricus is extracted by trichloroacetic acid to increase the permeability of the cell membrane, which is conducive to the better action of external substances on the cells and the discharge of cell contents, and then degreases to remove the fatty substances on the cell membrane, and uses enzymatic hydrolysis The complete peptidoglycan obtained by removing impurities in the cell after centrifugation and freeze-drying can be seen through transmission electron microscope observation that the cell wall skeleton structure is complete. Lactobacillus bulgaricus can be obtained from the public sale of Inner Mongolia Shuangqi Pharmaceutical Co., Ltd.
[0030] The preparation method of the complete peptidoglycan comprises the following steps:
[0031] (1) Cleaning and collection steps of bacteria:
[0032] Take 100 grams of Lactobacillus bulgaricus wet cells, add 1000 milliliters of normal saline, separate by centrifugation, centrifuge at 80...
Embodiment 2
[0045] Example 2: Structural integrity detection of a complete peptidoglycan of Lactobacillus bulgaricus disclosed by the present invention
[0046] The original thalline and the prepared complete peptidoglycan in Example 1 of the present invention are detected by electron microscopy, see figure 1 shown. The results showed that the complete peptidoglycan still retained the complete external structure of the original thalline.
Embodiment 3
[0047] Example 3: Anti-tumor effect test of a complete peptidoglycan of Lactobacillus bulgaricus disclosed by the present invention
[0048]Aseptically extract the peritoneal effusion from H22 liver cancer Kunming mice, trypan blue staining to check the survival rate of cancer cells, the peritoneal effusion tumor cells with a survival rate greater than 95% are adjusted to 1×107 cells / mL with serum-free medium . 40 normal Kunming mice were taken, half male and half male, and after adaptive feeding for 3 days, 2×106 cancer cells (0.2mL) were inoculated and injected subcutaneously in the right axilla of each mouse. One week later, the mouse liver cancer model was successfully established, and a tumor with a diameter of about 1 cm grew at the inoculation site. After modeling, the mice were randomly divided into 4 groups, 10 in each group, namely the control group (sterilized PBS1mL / d), the low-dose Lactobacillus bulgaricus intact peptidoglycan group (0.1mL / d), and the middle-dose...
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