Antineoplastic drug taking panaxadiol saponins as active ingredient and application thereof

A kind of anti-tumor drug, technology of notoginseng glycol, applied in the field of medicine

Inactive Publication Date: 2010-06-23
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report about the effect of notoginseng-diol group saponins (the active part isolated and extract

Method used

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  • Antineoplastic drug taking panaxadiol saponins as active ingredient and application thereof
  • Antineoplastic drug taking panaxadiol saponins as active ingredient and application thereof
  • Antineoplastic drug taking panaxadiol saponins as active ingredient and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The notoginsenodiol group saponins are dissolved in a small amount of DMSO, and then water for injection is added as usual, followed by precision filtration, potting and sterilization to prepare injections.

Embodiment 2

[0018] Notoginsengdiol group saponins, after dissolving with a small amount of DMSO, dissolve them in sterile water for injection, stir to dissolve, filter with a sterile suction funnel, and then aseptically filter, divide into ampoules, freeze at low temperature After drying, it is aseptically melt-sealed to obtain a powder injection.

[0019] In order to better understand the essence of the present invention, the following test examples of the present invention will be used to illustrate the results of the pharmacological effects of notoginsengdiol group saponins of the present invention, but the present invention is not limited by this test example.

experiment example 1

[0021] 1. Cell

[0022] Hela cells: human cervical cancer cells.

[0023] 2. Drugs and main reagents

[0024] Panax notoginseng diol group saponins were obtained from Kunming Pharmaceutical Group Co., Ltd., the primary antibody Anti-human Trx was provided by Professor Yodoi Yuji, Kyoto University, Japan; the primary antibody Anti-human CyclinD1 was purchased from Santa cruz; Protein Marker, purchased from Fermentas RPMI Medium 1640, purchased from Invitrogencorporation; Protein quantification kit (BCA method), purchased from Beyotime; Chemiluminesent substrate, purchased from Bio-Jingmei; PVDF membrane, purchased from Millipore; TRIS Base, purchased from NOVON; Acrylamide, purchased From Solarbio; sodium lauryl sulfate, purchased from xiamen sanlandchemicals company limited; agarose, Coomassie Brilliant Blue R-250, Aprotimin, PMSF and NP-40, all purchased from Sanland-chem International InC; TEMED, purchased from HUALVYUAN BIOTECHNOLOGY; X-ray film, purchased from China Lucky Film ...

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PUM

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Abstract

The invention relates to the technical field of medicine, and provides an antineoplastic drug taking panaxadiol saponins as active ingredient and an application of panaxadiol saponins for preparing the antineoplastic drug. The invention discloses panaxadiol saponins with antineoplastic activity, both trypan blue dye exclusion method and MTT method indicate that panaxadiol saponins can inhibit the growing of tumor cells; and protein immunoblotting method indicates that panaxadiol saponins can lower the expression level of thioredoxin and cyclins D1 in tumor cell.

Description

Technical field: [0001] The present invention relates to the technical field of medicine, in particular to an anti-tumor drug using notoginsenodiol group saponins as effective ingredients, and the application of notoginsenodiol group saponins in preparing anti-tumor drugs. Background technique: [0002] Panax notoginseng, also known as Tianqi and Panax notoginseng, is the rhizome of Panax notoginseng (Burk) F.H. Chen, a plant belonging to the genus Araliaceae. It is a rare and precious medicinal material of my country, mainly produced in Yunnan, Guangxi and other places. Panax notoginseng saponins are the main active components of Panax notoginseng. It has a wide range of pharmacological effects, such as anti-fatigue, improving hypoxia tolerance, lowering blood sugar, promoting blood circulation and removing blood stasis, improving immune function, scavenging free radicals, anti-inflammatory, and antioxidant. effect. In recent years, further research on notoginseng has shown tha...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P35/00A61K31/704
Inventor 白洁刘晓慧
Owner KUNMING UNIV OF SCI & TECH
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