Novel Bacillus amyloliquefaciens strain and application thereof

A technology of bacillus and liquefied starch, which is applied in the novel strain of bacillus amyloliquefaciens and its application field, can solve the problems of harming the human body and the environment, and achieve the effects of promoting health, reducing the amount of thrombus, preventing and treating cardiovascular diseases

Active Publication Date: 2013-02-27
农业药物毒物试验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional fungicides, bactericides and chemical synthetic agents not only inhibit fungal and bacterial infections, but also harm the human body and the environment

Method used

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  • Novel Bacillus amyloliquefaciens strain and application thereof
  • Novel Bacillus amyloliquefaciens strain and application thereof
  • Novel Bacillus amyloliquefaciens strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Characterization of novel Bacillus amyloliquefaciens

[0027] The novel Bacillus amyloliquefaciens (B. amyloliquefaciens Ba-BPD1) was screened by the inventor from the soil of Lishan, Taichung County, Taiwan, and further cultivated, identified and preserved. When culturing Bacillus amyloliquefaciens Ba-BPD1, a single colony thereof was inoculated and grown overnight in 6 ml of LB broth (Luria-Bertani, Miller, Difco). The cultured bacterial solution was then inoculated in 500ml LB culture medium at a ratio of 1 / 100, and cultured at 30°C and 150rpm for 6 days.

[0028] In addition, compared with other bacteria, Bacillus amyloliquefaciens Ba-BPD1 has a specific 16S ribosomal RNA sequence, and part of its 16S ribosomal sequence has been sequenced, and will be published in the US National Biotechnology Information on December 31, 2009 Central website (National Center for Biotechnology Information, NCBI, http: / / www.ncbi.nlm.gov / Genbank / ), its gene bank (GenBank)...

Embodiment 2

[0030] Example 2: Amylolytic enzyme produced by Bacillus amyloliquefaciens Ba-BPD1

[0031] In order to demonstrate that Bacillus amyloliquefaciens Ba-BPD1 can produce amylolytic enzyme (amylase) to hydrolyze starch, the amylase hydrolysis test is described below. A single colony of Bacillus amyloliquefaciens Ba-BPD1 was picked from a nutrient agar medium (nutrient agar (NA) plate) and mixed with 50 μl of sterile water as a bacterial solution. Then, drop this 50 μl bacterial liquid on a filter paper with a diameter of 1 cm, and place it on a yeast extract-soluble starch agar (yeast extract-soluble starch agar (YSA) plate, containing 1.0% yeast extract, 1.0% soluble starch and 1.5% agar). Place the YSA medium at 30°C for 2 to 3 days. After culturing, cover the YSA medium with 3 to 4ml iodine solution (containing 0.3% (w / v) iodine and 3% (w / v) potassium iodine (potassium iodine)), and measure the Ba- Colony size and clear zone size of BPD1. A blue-black coloration of the m...

Embodiment 3

[0033] Embodiment 3: the proteolytic enzyme produced by Bacillus amyloliquefaciens Ba-BPD1

[0034] To demonstrate that Bacillus amyloliquefaciens Ba-BPD1 produces proteolytic enzymes (proteases) to hydrolyze proteins, the proteolytic assay was described below. The bacterial liquid of Bacillus amyloliquefaciens Ba-BPD1 was prepared as described in Example 2. Drop 50 μl of bacterial liquid on a filter paper with a diameter of 1 cm, and place it on a skim milk agar (SMA) plate containing 1.5% skim milk powder, 1.3% nutrient broth (Nutrient broth, NB) and 1.5% agar ) on (Elsheikh, L, E., Bergman, R., Cryz, S.J.Jr. and Wretlind, B.A comparison of different methods for determining elastaseactivity of Pseudomonas aeruginosa strains from mink. Acta Pathol. Microbiol. Immunol. Scand. Sect. B , Microbiol. 1986. 94(3): 135-8). The SMA medium was cultured at 30° C. for 2 to 3 days, and the colony size and dissolution zone size of Bacillus amyloliquefaciens Ba-BPD1 were measured. The...

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Abstract

The invention relates to novel Bacillus amyloliquefaciens (Bacillus amyloliquefaciens Ba-BPD1) (Germany microbial culture collection center, the biological material sample collection number: DSM21836), which has a unique 16S ribosome RNA sequence named as SEO ID NO: 1 and can be used for manufacturing amylolytic enzyme, protease, cellulolytic enzyme, lipolysis enzyme and thrombus catabolic enzyme for displaying the biological degradation activity. The Bacillus amyloliquefaciens can also be used for producing antibiotics, such as iturin, fengycin and surfactin, and is used for inhibiting the growth of eumycete or bacteria. The novel Bacillus amyloliquefaciens and products thereof can be used for agriculture, sewage treatment, food industry and chemical industry.

Description

technical field [0001] The present invention relates to a novel strain of Bacillus amyloliquefaciens—Bacillus amyloliquefaciens, especially, the present invention relates to a novel strain of Bacillus amyloliquefaciens—Bacillus amyloliquefaciens Ba-BPD1 or its mutants, which are used to produce various enzymes and various antibiotics and biosurfactants. Background technique [0002] Microorganisms and their products have been widely used to improve human life, such as in food, wine, medicine, chemical industry and agriculture. These applications substantially reduce production and / or processing costs and satisfy human needs. [0003] Certain microorganisms produce enzymes to break down large molecules. For example, Yarrowia lipolytica (Yarrowialipolytica) produces lipid-decomposing enzymes (lipases) to degrade chemical oxygen demand (COD) in olive mill wastewater treatment (Lanciotti, R., Gianotti, A. , Baldi, D., Angrisani, R., Suzzi, G., Mastrocola, D. and Guerzoni, M.E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/24C12N9/54C12N9/42C12N9/16C12P19/14C12P21/00C12P21/06C02F3/34A23K1/16A01N63/02A01P21/00A01P3/00A61K35/74A61P31/04A61P31/10C12R1/07A61K35/742
Inventor 谢奉家高穗生
Owner 农业药物毒物试验所
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