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Homocysteine diagnosis/determination reagent (kit) and homocysteine concentration determination method

A homocysteine ​​and reagent technology, applied in the field of medical testing and determination, can solve the problems of complex operation, large variation of test data, and long test time.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Reduced coenzyme 0.25mmol / L

[0049] Betaine-homocysteine ​​methyltransferase 10000U / L

[0050] Methionine γ-lyase 9000U / L

[0051] Hydroxybutyrate dehydrogenase 10000U / L

[0052] Trimethylammonium acetate 5mmol / L

[0053] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0054] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested homocysteine ​​sample to the reagent is 1 / 25, the reaction direction is negative reaction (falling reaction), the delay time is about 0 minute...

Embodiment 2

[0057] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0058] Reagent 1

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 50mmol / L

[0061] Reduced coenzyme 0.25mmol / L

[0062] Trimethylammonium acetate 5mmol / L

[0063] Reagent 2

[0064] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0065] Stabilizer 500mmol / L

[0066] Betaine-homocysteine ​​methyltransferase 10000U / L

[0067] Methionine γ-lyase 9000U / L

[0068] Hydroxybutyrate dehydrogenase 10000U / L

[0069] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0070] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested homocysteine ​​sample and reagent 1, reagent 2 The volume rati...

Embodiment 3

[0073] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0074] Reagent 1

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 50mmol / L

[0077] Reduced coenzyme 0.25mmol / L

[0078] Trimethylammonium acetate 5mmol / L

[0079] Reagent 2

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Methionine γ-lyase 9000U / L

[0083] Hydroxybutyrate dehydrogenase 10000U / L

[0084] Reagent 3

[0085] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0086] Stabilizer 500mmol / L

[0087] Betaine-homocysteine ​​methyltransferase 10000U / L

[0088] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0089] When measuring the concentration of homocysteine, set it on the automatic biochemical analyzer: temperature 37°C, rea...

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Abstract

The invention relates to a homocysteine diagnosis / determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent compositions and components, which belong to the technical filed of medical test and determination. The reagent (kit) of the invention mainly includes: buffer solution, reduced coenzyme, trimethyl amino acetate salt, betaine-homocysteine methyltransgerase, methionine Gamma-catenase, hydroxybutyric acid dehydrogenase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet / visible light analyzer to detect the decreasing degree of absorbance at the 340nm position of a dominant wave so as to measuring and calculating the concentration of the homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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