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Method and device for establishing superficial sensory disturbance animal model

An animal model and shallow sensory technology, applied in the field of detection and recognition, can solve the problems of many subjective components, no recognized methods for evaluating shallow sensory disturbances such as pain and temperature, and rough assessment of sensory disturbances, so as to save operation time, reduce operation difficulty, The effect of easy operation

Inactive Publication Date: 2010-07-07
CHINA REHABILITATION RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] It can be seen that the clinical evaluation of sensory disturbances is rough and subjective, and in animal experiments, there is no recognized method for evaluating shallow sensory disturbances such as pain and warmth.

Method used

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  • Method and device for establishing superficial sensory disturbance animal model
  • Method and device for establishing superficial sensory disturbance animal model
  • Method and device for establishing superficial sensory disturbance animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Method for establishing animal model of shallow sensory impairment

[0056] The method for establishing an animal model of shallow sensory impairment provided in this embodiment involves operant conditioning based on animal avoidance of electric shocks, and the specific steps are as follows: figure 1 As shown, the details are as follows:

[0057] 1. Experimental animals: 40 healthy male Sprague-Dawley (SD) rats, weighing 249.90±2.03g, provided by the Animal Experiment Center of Beijing Academy of Military Medical Sciences. Under free light conditions, the room temperature is about 23°C, fed with fixed feed, and free to eat and drink.

[0058] 2. Experimental device: refer to Example 3.

[0059] 3. Specific steps:

[0060] (1) Establish an operant conditioned reflex for animals to avoid electric shocks

[0061] Put 40 rats into operant conditioning boxes surrounded by soundproof panels (see the schematic diagram of the structure) figure 2 ), the bottom ...

Embodiment 2

[0081] Example 2 Pathological examination

[0082] Intracerebral injection of biotin-labeled dextran (Biotin Dextran Amine, BDA) in healthy rats and hemiplegic rats with shallow sensory impairment after screening by the method described in Example 1, after feeding for 1 month, intraperitoneal injection (ip) Rats were anesthetized with 1% sodium pentobarbital (40 mg / kg). Use 300ml of normal saline (37°C) to wash out the blood in the blood vessels when perfusion, and then perfuse and perfuse overnight (4°C) after the liver and cornea turn white. Light microscopy specimens are perfused and fixed with 4% paraformaldehyde; electron microscopy specimens are perfused with 2 % paraformaldehyde and 2% glutaraldehyde for perfusion and fixation. The perfusion was first fast and then slow. After 30 minutes, the limbs of the rats were stiff, and the brain was removed by craniotomy. After frozen sections, light and electron microscope observations were carried out.

[0083] Light micros...

Embodiment 3

[0086] Example 3 Apparatus for the method described in Example 1

[0087] The device proposed by the present invention, which is applied to the method for establishing an animal model of shallow sensory impairment described in Example 1, consists of a conditional reaction box 1 and a circuit control box. figure 2 It is the structural representation of the conditional reflection box of the device, wherein said conditional reflection box 1 comprises the following parts:

[0088]The electric shock device 2 is composed of copper rods, and an elongated welding circuit board is arranged outside it, and the copper rods are welded on the circuit boards and fixed on the bottom surface of the box body. Within the range of animal activities, the voltage is alternating current, and there are five levels of voltage that can be adjusted, namely 33v, 36v, 39v, 42v, 45v. There are manual switches that need to be adjusted manually.

[0089] The joystick covers the cover box 3, including a ...

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Abstract

The invention provides a method and a device for establishing a superficial sensory disturbance animal model. The method comprises the following steps: (1) establishing operant conditioned reflex avoiding noxious stimulation for animals; (2) preparing hemiplegia models for animals which establish conditioned reflex; and (3) only exposing the affected limbs of hemiplegia animals, and screening superficial sensory disturbance. The device is suitable for the method for establishing the operant conditioned reflex avoiding shock stimulation for animals, which comprises a box body, a circuit control device, and a shock device and a power-off device which are positioned at the bottom of a box. The method for establishing the superficial sensory disturbance animal model provided by the invention can accurately record the performance of animals in the process of experiments in detail and facilitate the collation and statistics of data, can establish a superficial sensory disturbance model of affected limbs of the animals after stroke, has the advantages of simple operation, accurate data, easy training for animals, short test cycle and the like; and the corresponding device has simple structure and easy operation.

Description

technical field [0001] The invention relates to the field of detection and identification technology methods, and relates to a method and a device for establishing an animal model of shallow sensory impairment based on animal operant conditioning. Background technique [0002] In 1903, the Russian physiologist I.P. Pavlov discovered the phenomenon of conditioned reflex through animal experiments and proposed the theory of conditioned reflex. His main interest was the study of digestive activity in dogs, initially by observing dogs salivating profusely when they craved food. He thinks it's unlikely that dogs salivating before they're actually given food is an innate response. There is good reason to think that by this time the animal has learned to associate a certain signal or the smell of the food with the food itself, and that salivation is due to this learning. So in craving food, the dog salivates in response to a new stimulus that heralds the imminent arrival of food,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
Inventor 张通杨宇琦公维军
Owner CHINA REHABILITATION RES CENT
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