Multifunctional EPR bio-probe based on magnetic nano particle and application thereof
A technology of magnetic nanoparticles and biological probes, applied in the field of multifunctional EPR biological probes based on magnetic nanoparticles, can solve problems such as limited application and achieve excellent dispersion effect
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Embodiment 1
[0036] Weigh 2.16g FeCl 3 ·6H 2 O was dissolved in a 50ml round bottom flask by adding 20ml of double distilled water, and then used after ultrasonication. Take another 50ml round bottom flask, weigh 4.20g of citric acid and add 10ml of double distilled water, stir magnetically for 30min and set aside. Add 1.12g FeSO to a 250ml four-neck flask 4 ·7H 2 O, and add 20ml of double-distilled water to dissolve it, heat to 50°C under mechanical stirring, and deoxygenate with nitrogen for 30min. Then add FeCl to be used 3 Solution, and heated to 70 ° C, after reaching the reaction conditions, slowly add 5 ~ 15ml of ammonia water drop by drop, aging for 30min after the dropwise addition. Then increase the stirring intensity, add the citric acid (CA) solution to be used at one time, react for 1.5h, cool naturally and separate the particles with a magnet. Weigh 10 mg of the above particles into a 50 ml round bottom flask, add 10 ml of double distilled water, and stir magnetically i...
Embodiment 2
[0038] Weigh 1.08g FeCl 3 ·6H 2 O was dissolved in a 50ml round bottom flask by adding 20ml of double distilled water, and then used after ultrasonication. Take another 50ml round-bottom flask, weigh 2.08g of malonic acid and add 10ml of double distilled water, stir magnetically for 30min and set aside. Add 0.56g FeSO to a 250ml four-neck flask 4 ·7H 2 O, and add 20ml of double-distilled water to dissolve it, heat to 40°C under mechanical stirring, and deoxygenate with nitrogen for 30min. Then add FeCl to be used 3Solution, and heated to 80 ° C, after reaching the reaction conditions, slowly add 5 ~ 15ml of ammonia water drop by drop, aging for 30min after the dropwise addition. Then increase the stirring intensity, add the ready-to-use malonic acid (MA) solution at one time, react for 1.5 h, cool naturally and separate the particles with a magnet. Weigh 10 mg of the above particles into a 50 ml round bottom flask, add 10 ml of double distilled water, and stir magnetical...
Embodiment 3
[0040] Kunming mice (about three weeks old, with an average body weight of 10 g during the test) were randomly divided into 5 groups (N=3), and each group was injected with a certain dose of modified magnetic nanoparticles (Fe 3 o 4 -CA-4-NH 2 -TEMPO) suspension, the intervals were 0.5 hour, 1 hour, 2 hours, and 3 hours to kill the mice, and blood, heart, liver, spleen, lung, and kidney samples were taken respectively. Anticoagulants were added to the blood samples, and the serum was collected by centrifugation; 50% tissue homogenates were made from the remaining organs. After sampling, the tissue homogenate was stored on ice until EPR testing; a group was reserved for long-term observation.
[0041] Through observation, it was found that the mice had no abnormal reaction after one, two, and three weeks. Preliminary tests show that the material is less toxic, and the samples are injected subcutaneously, and the mice have no abnormal behavior within one month; the in vivo di...
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