Primers and probes for carrying out florescent quantitive detection of hepatitis C virus
A quantitative detection and probe technology, applied in biochemical equipment and methods, microbial measurement/testing, microorganisms, etc., can solve problems such as sequence differences and difficulty in diagnosis and detection
Inactive Publication Date: 2010-08-25
JIANGSU MOLE BIOSCI
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There are significant sequence differences between different HCV virus isolates, and multiple genotypes make diagnostic detection difficult
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The invention discloses primers and probes for carrying out florescent quantitive detection of hepatitis C virus, which are used for detecting florescent quantitive PCR (Polymerase Chain Reaction) at the real time. The primers and the probes have the advantages of high specificity and high augmentation efficiency. Therefore, the invention greatly improves the sensitivity and the specificity of the HCV RNA detection. A design method of the primers and the probes comprises the following steps of: comparing all HCV complete genomes in a GenBank by adopting a comparison software, identifying a plurality of main conversation areas, then further screening by adopting blast, and designing a plurality of primers and probes.
Description
Primers and Probes for Fluorescent Quantitative Detection of Hepatitis C Virus Technical field: The present invention relates to multiple primers and probes with high specificity and high amplification efficiency for detecting hepatitis C virus ribonucleic acid. Background technique: Hepatitis C virus (HCV) is a non-gastrointestinal virus that causes post-transfusion hepatitis disease. It is estimated that more than 1% of the global population is infected with HCV. Acute and chronic hepatitis, cirrhosis and hepatocellular carcinoma are all associated with HCV infection. . At present, HCV is classified as an independent genus in the Flaviviridae family, and its genome is characterized by a single positive-stranded RNA molecule of about 9500 bp, containing a protein precursor ORF that encodes about 3000 amino acids. The most conserved 340bp 5'UTR in the ORF 5' region encodes a capsid protein, two envelope glycoproteins (E1 and E2) and a small protein of unknown function (P7...
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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
Inventor 杨英符芳芳李杨霞
Owner JIANGSU MOLE BIOSCI