Bactericidal composition
A technology of compositions and derivatives, applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of drug resistance, decline in control effect, inhibition of crop growth, etc., to reduce pollution, reduce dosage, and reduce drug dosage. Effect
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example 1
[0018] Bioassay example 1: Indoor toxicity test of prochloraz and hexaconazole compound against wheat powdery mildew
[0019] Test object: wheat powdery mildew (collected from the field and cultivated indoors)
[0020] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1156.4-2006", pot method. Select three-to-four-leaf potted wheat seedlings with consistent growth vigor, and select three pots of wheat seedlings for testing for each treatment, numbered for future use. Wheat powdery mildew diseased leaves collected from the field were evenly shaken off the conidia above the wheat seedlings for inoculation, and 24 hours later, they were treated with chemicals. See Table 1 for the test chemicals. Use a spray tower to spray under 50PSI pressure, 5mL per treatment. Five concentration gradients were set for each agent, and the same amount of water sprayed was used as the blank control. After spraying, the wheat seedlings were put into the greenho...
example 2
[0031] Biological Assay Example 2: Indoor Toxicity Determination of Prochloraz and Hexaconazole Compound to Botrytis cinerea
[0032] Test object: Botrytis cinerea (collected from the field and separated and purified)
[0033] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1156.2-2006", mycelium growth rate method. Cucumber Botrytis cinerea was cultivated with PDA medium. When the bacterial colony just overgrown the petri dish, a puncher with an inner diameter of 5 mm was punched from the edge, and the resulting mycelium block was used as an inoculum. Take 5 mL of the prepared drug solution and mix with quantitative 75 mL of sterilized medium to make 4 drug-containing plates, and use the same amount of sterile water mixed with the medium as the control. Put the mycelium block upside down and connect it to the center of the plate, and cultivate it in a constant temperature incubator at 28°C. After 5 days, the colony diameter was measured ...
example 1
[0036] The mycelial growth inhibition rate was converted into a probability value (y), the concentration of the drug solution (μg / ml) was converted into a logarithmic value (x), and the toxicity equation and the inhibition concentration EC were calculated by the least square method 50 According to Sun Yunpei's method, the toxicity index and co-toxicity coefficient (CTC) of the drug were calculated. The calculation method is the same as the biological assay example 1. The results of the toxicity test are shown in Table 2.
[0037] The results in Table 2 show that when the ratio of prochloraz and hexaconazole is between 50:1 and 1:20, it has a significant synergistic effect on cucumber gray mold, especially between 20:1 and 1:5. time, the synergistic effect is more obvious, and the co-toxicity coefficient is above 180.
[0038] Table 2 Indoor toxicity test results of prochloraz and hexaconazole against Botrytis cinerea
[0039] deal with
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