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Method and application for extracting alexandrium tamarense inhibitor from enteromorpha
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A technology of inhibitor and red tide algae, applied in the field of seawater treatment, can solve the problems of marine ecological environment loss, seawater eutrophication, aquatic animal and human health hazards, etc., achieving good ecological safety, growth inhibition, and easy operation and control. Effect
Inactive Publication Date: 2013-05-08
秦旭坤
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Abstract
Description
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Problems solved by technology
In addition, due to land-based nutrients and pollutants discharged and leaked from marine operations, a large amount of pollutants enter the ocean, resulting in eutrophication of seawater
Eutrophication leads to the explosive growth of red tide algae, which brings huge losses to the marine ecological environment, aquaculture industry, tourism industry, etc.
Some algae can also produce algae toxins, which are harmful to aquatic animals and human health
Method used
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Effect test
Embodiment 1
[0017] Take 0.1 kg of Enteromorpha soaked in 0.3 liters of distilled water for 24 hours, then filter, and concentrate the filtrate.
[0018] Dissolve the concentrated paste with 40 ml of a 1:1 mixture of petroleum ether and 90% ethanol. Shake well, stand still for layering, and remove the lower layer (ethanol phase). The ethanol phase was concentrated by evaporation under reduced pressure at 40° C. to obtain a paste-like substance, which was extracted and separated with 20 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (approximately 10 ml).
[0019] 0.1, 0.2, 0.3, 0.4, 0.5 ml of inhibitors were added to 200 ml of the typical red tide algae Gymnodinium breve culture solution. After 3 days, the inhibition rates were 65%, 86%, 96%, 98%, and 99.5%.
Embodiment 2
[0021] One kilogram of Enteromorpha was soaked in 2 liters of distilled water for 48 hours, then filtered, and the filtrate was concentrated.
[0022] Dissolve the concentrated paste with 100 ml of a 1:1 mixture of petroleum ether and 90% ethanol. Shake well, stand still for layering, and remove the lower layer (ethanol phase). The ethanol phase was concentrated by evaporation under reduced pressure at 40°C to obtain a paste-like substance, which was extracted and separated with 100 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (approximately 50 ml).
[0023] 0.2, 0.3, 0.4, 0.5, 1 ml of inhibitors were added to 100 ml of the typical red tide algae Gymnodinium breve culture solution. After 3 days, the inhibition rates were 81%, 92%, 97%, 99%, and 99%.
Embodiment 3
[0025] 1 kg of Enteromorpha was soaked in 5 liters of distilled water for 24 hours and then filtered, and the filtrate was concentrated.
[0026] Dissolve the concentrated paste with 100 ml of a 1:1 mixture of petroleum ether and 90% ethanol. Shake well, let stand for layering, and remove the ethanol phase of the lower layer. The ethanol phase was concentrated by evaporation under reduced pressure at 40°C to obtain a paste-like substance, which was extracted and separated with 100 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (approximately 50 ml).
[0027] 0.2, 0.3, 0.4, 0.5, 1 ml of inhibitors were added to 100 ml of the typical red tide algae Gymnodinium breve culture solution. After 2 days, the inhibition rates were 76%, 82%, 87%, 95%, and 97%.
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Abstract
The invention relates to a method and an application for extracting an alexandrium tamarense inhibitor from enteromorpha. The method comprises the following steps of: lixiviating per kilogram of the enteromorpha by using 2-5 liters of distilled water at room temperature for 12-72 hours, filtering to take filter liquor, and concentrating the obtained filter liquor into pastes by adopting a rotary evaporation method; dissolving the concentrated pastes by using a mixing solution of petroleum ether and 90 percent of alcohol which are in a 1:1 ratio; sufficiently oscillating for 1-3 minutes, then demixing by standing for 10-120 minutes, and taking a lower-layer alcohol phase; decompressing, evaporating and concentrating the alcohol phase to obtain a paste-like substance at 40 DEG C; extractingand separating by using ethyl acetate and secondary distilled water which are in the 1:1 ratio, wherein a ethyl acetate phase is the alexandrium tamarense inhibitor; and determining the volume of thealexandrium tamarense inhibitor extracted by per kilogram of the enteromorpha to 50 milliliters. The invention is used for inhibiting the growth of alexandrium tamarense by adding 0.5-5 milliliters of the alexandrium tamarense inhibitor to per cubic metre of a water body, sufficiently utilizes the enteromorpha, has efficient economy, good ecological safety and easy operation and control, effectively inhibits the growth of the oceanic alexandrium tamarense and particularly inhibits the growth of gymnodinium breve.
Description
Technical field [0001] The invention belongs to the field of seawater treatment, and in particular relates to a method and application for extracting red tide algae inhibitors from Enteromorpha. Background technique [0002] Enteromorpha prolifra (Muell.) J.Ag., also called moss strips and moss, is a common large seaweed. The algae body is grass green, tubular membranous, clumped, with obvious main branches, slender branches, up to 1 meter high. Beginning in 2006, a large number of Enteromorpha species gather and float on the surface of the Yellow Sea every summer, forming large areas of green tides in northern Jiangsu and the southern coast of the Shandong Peninsula. Especially in June 2008, a large amount of Enteromorpha drifted from the central waters of the Yellow Sea to the waters near Qingdao. The coastal waters and coasts of Qingdao encountered sudden and historically rare natural disasters of Enteromorpha. At its peak, the distribution area reached 24,000 square kilomet...
Claims
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Application Information
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