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Method for collecting semen from cynomolgus monkey

A technology for cynomolgus monkeys and semen collection, which is applied in the fields of medical science, veterinary instruments, animal delivery, etc., and can solve the problem of not finding the same or similar reports.

Inactive Publication Date: 2010-10-13
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Have not found so far identical or similar report with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] Select 5-8 adult healthy male cynomolgus monkeys, and two breeders will grasp the male monkeys by their limbs, fully expose the genitals, and appease the cynomolgus monkeys appropriately to stabilize their emotions.

[0016] One keeper cleans the penis and places electrodes, another technician takes a 50ml sterilized centrifuge tube to receive the semen, and is responsible for adjusting and switching on and off the physiological and pharmacological electronic stimulator, and the operator wipes the cynomolgus monkey with a wet towel or gauze in saline The penis was cleaned several times in order to clean the penis of the cynomolgus monkey and enhance the conductivity. Two self-made penile electrodes, one connected to the positive pole and the other connected to the negative pole, were respectively fixed on the front part of the penis body and the back part of the penis body of the cynomolgus monkey , and adjust the ring size of the self-made penis electrode according to t...

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PUM

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Abstract

The invention discloses a method for collecting semen from a cynomolgus monkey. The method adopts a physiological and pharmacological electronic stimulation instrument, two self-made penis electrode devices, a strictly sterilized centrifugal tube, a pipette and a thermos flask; and the semen collecting method adopts a non-narcotic restraint method. An operator collects the semen through the following steps of: 1, soothing the cynomolgus monkey to stabilize the mood thereof; 2, sterilizing the appliances and the penis of the cynomolgus monkey; and 3, turning on a power supply of the physiological and pharmacological electronic stimulation instrument to perform simulation so as to collect the semen. The method has the advantages that the method can be used for artificial insemination of the cynomolgus monkey and normal-temperature and freezing preservation of the semen, can fully play the reproductive efficiency of excellent male cynomolgus monkeys, can preserve genetic genes of the excellent cynomolgus monkeys and meanwhile provides experience reference for the semen collecting technology for other rare endangered primates.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for semen collection of cynomolgus monkeys. Background technique [0002] So far, no report identical or similar to the present invention has been found. Contents of the invention [0003] The purpose of the present invention is to provide a method for semen collection in cynomolgus monkeys. [0004] The technical scheme that the present invention solves the problems of the technologies described above is as follows: [0005] A semen collection method for cynomolgus monkeys, the method adopts one physiological and pharmacological electronic stimulator, two self-made penis electrodes; a strictly sterilized centrifuge tube; method, the specific steps are as follows: [0006] 1) 1-2 persons hold the male monkey in the cage by its limbs, fully expose the genitals, and properly appease the cynomolgus monkey to stabilize its mood; [0007] 2) There are tw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00
Inventor 卢晟盛卢克焕李林
Owner GUANGXI UNIV
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