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Heterotrophic nitrification aerobic denitrifying bacteria and culture method and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of highly efficient heterotrophic nitrification aerobic denitrification bacteria and its cultivation, can solve the problems of increasing difficulty, affecting the stability of denitrification effect of wastewater biological treatment system, and large energy consumption question

Active Publication Date: 2010-11-03
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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Problems solved by technology

[0004] The process is lengthy, because the process conditions of nitrification and denitrification are different, they need to be completed in two systems respectively, resulting in two deficiencies
First of all, it consumes a lot of energy. Ammonia nitrogen nitrification requires oxygen consumption, that is, it needs to consume energy to supply oxygen. The pre-denitrification system needs to set a relatively large reflux in the mixed liquid, which also increases energy consumption.
Secondly, the denitrification reaction must have a carbon source as an electron donor. If the carbon source in the sewage is insufficient (C / N is too low), then organic carbon such as methanol needs to be added, which not only increases the operating cost, but also increases the operational management and maintenance costs. The difficulty of subsequent processing
Therefore, the investment in wastewater treatment projects is large and the operating costs are high.
Moreover, nitrifying bacteria are usually autotrophic nitrifying bacteria, which proliferate slowly and are easily eliminated in the wastewater biological treatment system, thus affecting the stability of the denitrification effect of the wastewater biological treatment system

Method used

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  • Heterotrophic nitrification aerobic denitrifying bacteria and culture method and application thereof
  • Heterotrophic nitrification aerobic denitrifying bacteria and culture method and application thereof
  • Heterotrophic nitrification aerobic denitrifying bacteria and culture method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0071] Embodiment 1: the cultivation of bacterial strain of the present invention

[0072] (1) Beef extract medium and organic carbon source denitrification medium, sterilized at 121°C for 20 minutes.

[0073] (2) The strain Rhodococcus DN2.3 was inoculated onto the sterilized beef extract slope, activated and cultivated at 37°C for 24-36 hours for later use.

[0074](3) Inoculate the activated strains in beef extract liquid medium or organic carbon source denitrification liquid medium, fill 50mL medium in a 250mL Erlenmeyer flask, culture at 37°C and shake at 170-200rpm for 24-36h.

[0075] (4) 4000rpm, 10min centrifugation to harvest the bacteria or directly transfer the bacteria suspension to the wastewater sample to be treated.

Embodiment 2

[0076] Embodiment 2: bacterial strain of the present invention is to ammonia nitrogen and COD under the condition that sodium acetate is organic carbon source Cr removal of

[0077] Simulated wastewater (g / L): KH 2 PO 4 0.7, MgSO 4 ·7H 2 O 0.5, CaCl 2 2H 2 O 0.5, (NH 4 ) 2 SO 4 0.5, sodium acetate 2.6, NaOH to adjust the pH to 7.2-7.4, and sterilize at 121°C for 20 minutes.

[0078] Preparation of simulated wastewater with sodium acetate as carbon source and ammonium sulfate as nitrogen source, the initial COD Cr About 2000mg / L, ammonia nitrogen about 100mg / L. Inoculate 2% of the bacterial suspension (see Example 1 for the culture method), replace it with the same volume of sterilized water, 37 ° C, 170 rpm shaking culture, and regularly take samples to measure the changes in the concentration of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen. The results are shown in Table 1. It can be seen from Table 1 that when sodium acetate is the organic carbon so...

Embodiment 3

[0081] Embodiment 3: bacterial strain of the present invention is to ammonia nitrogen and COD under the condition that glucose is organic carbon source Cr removal of

[0082] Simulated wastewater (g / L): KH 2 PO 4 0.7, MgSO 4 ·7H 2 O 0.5, CaCl 2 2H 2 O 0.5, (NH 4 ) 2 SO 4 0.5, glucose 2.0, adjust the pH to 7.2-7.4 with NaOH, and sterilize at 121°C for 20 minutes.

[0083] Preparation of simulated wastewater with glucose as organic carbon source and ammonium sulfate as nitrogen source, the initial COD Cr The concentration is 2000mg / L, and the ammonia nitrogen is about 100mg / L. Inoculate 2% bacterial suspension (see Example 1 for the culture method), replace the control with the same volume of sterilized water, culture at 30°C with shaking at 200rpm, and measure COD regularly Cr and ammonia nitrogen content. The experimental results are shown in Table 2. As can be seen from Table 2, DN2.3 in the initial COD Cr When the concentration is 2000mg / L, culture for 48h, a...

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Abstract

The invention belongs to the technical field of environmental microbiology, and relates to high-efficiency heterotrophic nitrification aerobic denitrifying bacteria and a culture method and application thereof. The bacteria are Rhodococcus sp. DN2.3 with the preservation registration number of CCTCC M209300, can effectively remove ammonia nitrogen, nitrite nitrogen, nitric nitrogen and the mixture thereof from a water body, also can remove CODCr from organic wastewater, are suitable for treating high-concentration organic nitrogen-containing wastewater; the accumulation of nitrite and nitrate is avoided in the denitrifying process; and the process for treating wastewater by using the strain is simple and the denitrifying effect is stable.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a high-efficiency heterotrophic nitrification-aerobic denitrification bacterium and its cultivation method and application. Background technique [0002] Nitrogen-containing wastewater is a major problem in environmental pollution control. Biological denitrification is currently recognized as one of the most economical and effective methods for denitrification of wastewater. The traditional ammonia nitrogen wastewater treatment is to convert ammonia nitrogen into nitrogen gas through the combined process of nitrification by autotrophic nitrifying bacteria and denitrification by heterotrophic denitrifying bacteria. [0003] [0004] The process is tedious, and due to the different process conditions of nitrification and denitrification, it needs to be completed in two systems respectively, resulting in two deficiencies. First of all, it consumes a lot o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01C02F101/16
CPCY02W10/10
Inventor 黄钧牟丽娉赵永贵
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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