Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Leachii mycoplasma Chinese isolate and isolation medium and application thereof

A technology of mycoplasma and isolates, which is applied in the field of mycoplasma isolates, the development of biological products for the prevention and control or detection of diseases caused by Leachii mycoplasma, and achieves the effects of reducing production costs, shortening the cultivation period, and reducing side effects

Inactive Publication Date: 2010-11-10
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, no isolates of Mycoplasma Leachii have been isolated in China. Therefore, the isolation of a Mycoplasma Leachii isolate in China is of great importance for the study of the transmission route of Mycoplasma Leachii and the development of biological products for the prevention or treatment of diseases caused by Mycoplasma Leachii significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Leachii mycoplasma Chinese isolate and isolation medium and application thereof
  • Leachii mycoplasma Chinese isolate and isolation medium and application thereof
  • Leachii mycoplasma Chinese isolate and isolation medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] 1. Test materials and methods

[0034] 1. Sample source

[0035] From February to June 2009, severe polyarthritis occurred in more than 300 female calves in a dairy farm in northern China, from which 2 joint fluids of calves with early onset and typical symptoms were aseptically collected, -70 ℃ frozen for later use.

[0036] 2. Test material

[0037] DNA Gel Recovery Kit, Column Small Tissue / Cell Genomic DNA Extraction Kit; Ex Taq DNA Polymerase; Hyclone Donor Horse Serum; PPLO Broth (BD-Difco); Yeast Infusion (BD-Bacto) ; Other chemical reagents used in the experiment were of analytical grade.

[0038] 3. Main instruments and equipment

[0039] Pipettes of various specifications and 5415R, 5810R high-speed desktop low-temperature centrifuges (Eppendorf, Germany); MCO-15AC carbon dioxide incubator, MDF-U32V ultra-low temperature refrigerator (SANYO, Japan); IX51 inverted microscope (OLYMPUS); Safety cabinet (LABCONCO, USA); DK-8D electric heating constant temperat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Leachii mycoplasma Chinese isolate and an isolation medium and application thereof. The microbe preservation number of the Leachii mycoplasma Chinese isolate isolated by the invention is CGMCC No.3808. The invention also discloses an isolation or subculture medium suitable for the Leachii mycoplasma Chinese isolate. Pathogenicity test results prove that through inoculating the joints of a healthy calf which is 1-2 month old with the subculture of the mycoplasma isolate, typical polyarthritis can be copied and a certain death can be caused; the dead calf has obvious general symptom, and multiple tissues and organs have obvious pathological changes. The mycoplasma isolated by the invention is the Leachii mycoplasma, which is confirmed by combining with clinical morbidity and the clinical symptoms and the pathological changes of artificially inoculated calf, the epidemical characteristics of the disease, the traceable epidemiological survey and the specific PCR (Polymerase Chain Reaction) identification and the genetic evolution analysis of an isolate pathogen.

Description

technical field [0001] The present invention relates to a strain of mycoplasma (Mycoplasma) isolate, relate in particular to the Chinese isolate of Leachii mycoplasma and the culture medium that is suitable for this isolate and subculture, the present invention also relates to this isolate in the research and development of prevention and control or detection by Leachii mycoplasma The use in disease-causing biological products belongs to the field of separation and application of mycoplasma. Background technique [0002] Mycoplasma (Mycoplasma) is a class of minimal prokaryotic microorganisms that lack cell walls, are highly pleomorphic, can pass through bacteria filters, and can grow and reproduce in non-living media. Mycoplasma was first isolated by Nocard et al. in 1898, and was officially named Mycoplasma in 1956. A new independent class - Mollicutes was established in the microbial classification, and it became an independent discipline, that is, mycoplasma. [0003] M...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04A61P19/02A61P11/00G01N33/569C12R1/35A61K35/741
Inventor 于力常继涛刘海军
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com