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Specific PCR molecular markers for detecting rice blast resistance alleles

A technology of resistance to rice blast and alleles, applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc.

Inactive Publication Date: 2010-11-10
CHINA NAT RICE RES INST
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Problems solved by technology

In general, rice blast-resistant varieties and high-yielding varieties are often crossed to obtain high-yielding blast-resistant varieties. In the backcross breeding for transferring disease-resistant genes, the identification of panicle blast resistance must be completed before heading. The conventional panicle blast identification must It can only be carried out after earing, and this condition cannot be met

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  • Specific PCR molecular markers for detecting rice blast resistance alleles
  • Specific PCR molecular markers for detecting rice blast resistance alleles
  • Specific PCR molecular markers for detecting rice blast resistance alleles

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[0009] Such as figure 1 As shown, A. the detection result of molecular marker Si13068; B. the detection result of molecular marker Si13070B2; C. the detection result of molecular marker Si13070C; D. the detection result of molecular marker Si13070D. G: Gumei No. 2; 1: II-32B; 2: Dyou 62B; 3: V20B; 4: Bo B; 5: Chuanxiang 29A; 6: Feigai B; 7: Fengyuan A; 8: Gang 46B ;9: Jin 23B; 10: Longtepu B; 11: Neixiang 2A; 12: Neixiang 85A; 13: Tianfeng A; 14: Xieqingzao B; 15: Yin 32B; 16: You IB; Zhenshan97B; 18: Zhong100A; 19: Zhong1A; 20: Zhong2B; 21: Zhong3B; 22: Zhong9B; 23: ZhongzheA; 24:207; 25:253; 26:926: 27: CDR22 ;28: R402; 29: To974; 30: ZDZ057; 31: Ce 64; 32: Duoxi No. 1; 33: Enhui 58; Jianghui 151; 38: Luhui 17; 39; Miyang 46; 40: Mianhui 501; 41: Mianhui 725; 42: Minghui 63; 43: Minghui 70; 44: Minghui 77; 45: Minghui 86; 46: Shuhui 162; 47: Shuhui 527; 48: Yanhui 559; 49: Yihui 1577; 50: Zhenhui 084; 51: CH238; 52: CH59; 53: Zhonghui 218; 54: Zhonghui 8006; 55: Zhonghui...

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Abstract

The invention provides four specific PCR markers for detecting rice blast resistance alleles, which are Si13068, Si13070B2, Si13070C and Si13070D respectively and characterized by PCR primers thereof. The upstream primer of the Si13068 is 5'-TCTTAAAGGAGCCGTCT-3', and the downstream primer is 5'-GAAACTTAAACTGTACGTGTG-3'; the upstream primer of the Si13070B2 is 5'-TTATGTAAGAAGCTAGATCCGTA-3', and the downstream primer is 5'-AGAGCTGTTTATGTCGACCA-3'; the upstream primer of the Si13070C is 5'-ATAACGTGTGTCCATAACAGT-3', and the downstream primer is 5'-TTAATGACTCGTGTATAGAGC-3'; and the upstream primer of the Si13070D is 5'-CAAGTTTTACTCTCCCGTT-3', and the downstream primer is 5'-CCAGCTAGATTAATGACTCG-3'. The utilization of the four markers for carrying out identification on DNA extracted during the rice seedling stage can detect whether a material to be detected is transferred into Gumei No.2 Pi25 rice blast resistance alleles or not, and detect whether the material has leaf blast resistance capacity and panicle blast resistance capacity or not, and the whole detection process has simple operation and high accuracy.

Description

technical field [0001] The invention relates to the technical field of detection in rice breeding, in particular to a specific PCR marker for detection of Pi25 blast resistance alleles of Gumei No. 2. Background technique [0002] Rice blast is one of the most important rice diseases caused by Ascomycete Magnaporthe grisea Barr, which widely occurs in various rice regions in the world, and panicle blast has a particularly serious impact on rice yield. Planting blast-resistant rice varieties is the most cost-effective means to solve this problem. Generally, rice blast-resistant varieties and high-yielding varieties are often crossed to obtain high-yielding blast-resistant varieties. In the backcross breeding for transferring disease-resistant genes, the identification of panicle blast resistance must be completed before earing. Conventional panicle blast identification must Can carry out after earing, can't satisfy this condition. With the rapid development of molecular mar...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 朱玉君庄杰云樊叶杨应杰政
Owner CHINA NAT RICE RES INST