Test kit for identifying single nucleotide polymorphism of target nucleic acid

A single nucleotide polymorphism and nucleic acid technology, applied in the field of test kits for identifying single nucleotide polymorphisms of target nucleic acids, can solve the problem that polymorphisms cannot fully explain the difference in warfarin dosage requirements, Low frequency and other issues

Inactive Publication Date: 2010-11-17
PHARMIGENE
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low frequency of these two CYP2C9 variants in Asians, polymorphisms in this gene cannot fully explain the differences in warfarin dosage requirements, es

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Test kit for identifying single nucleotide polymorphism of target nucleic acid
  • Test kit for identifying single nucleotide polymorphism of target nucleic acid
  • Test kit for identifying single nucleotide polymorphism of target nucleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention proposes a test set that can be used to identify single nucleotide polymorphisms of target genes, especially a test set that can identify single nucleotide polymorphisms of target genes including CYP2C9 gene and VKORC1 gene in biological samples Test suite.

[0019] Accordingly, the inventor proposes a probe group suitable for determining single nucleotide polymorphisms of target genes including the CYP2C9 gene and the VKORC1 gene, including the first, second and third groups of probes; and A pair of primers for PCR reaction for incrementally amplifying the detected target nucleic acid includes first, second and third primer pairs. Using the first, second and third groups of probes and the first, second and third primer pairs respectively in combination to detect a single nucleotide polymorphism of G>A at position -1639 of the VKORC1 gene in a biological sample sex, the single nucleotide polymorphism of A>C at position 1075 of the CYP2C9 gene, or ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a test kit for identifying single nucleotide polymorphism of target genes including a CYP2C9 gene and a VKORC1 gene in a biological sample. The kit comprises a first group of probes and a second group of probes, wherein a first probe in the first group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:3, 4 and 5 and a complementary sequence thereof is at least 80 percent; a second probe in the first group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:6, 7 and 8 and a complementary sequence thereof is at least 80 percent; a first probe in the second group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:11, 12 and 13 and a complementary sequence thereof is at least 80 percent; and a second probe in the second group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:14, 15 and 16 and a complementary sequence thereof is at least 80 percent. The reagent kit can also comprise a first primer pair and a second primer pair.

Description

technical field [0001] The present invention is generally related to determining the appropriate starting dose or suitable dose for each individual based on the simultaneous two genotypes of the individual. Specifically, the present invention is through a test kit, especially a test kit for identifying single nucleotide polymorphisms of target genes including CYP2C9 gene and VKORC1 gene in a biological sample, To identify the single nucleotide polymorphisms of at least two target nucleic acids in this individual, and then determine the appropriate starting dose or applicable dose for this individual. Background technique [0002] A group of mutated single nucleotides at the positions of the gene sequence, also known as "single nucleotide polymorphisms (SNPs)", are distributed throughout the gene sequence. A single nucleotide polymorphism can also be allelic, that is, due to the existence of a genetic polymorphism, some individuals in a species will have a non-variant sequen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 许仁祺林心郁李怡侬
Owner PHARMIGENE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap