Complex microbial inoculum for swine production by fermentation bed started quickly at low temperature and preparation method thereof

A compound microbial inoculum, a fast-start technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of shortage, high cost of raising pigs in fermentation beds, unstable production, etc. The effect of solving environmental pollution and heating up quickly

Active Publication Date: 2010-12-15
甘肃绿能农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the 1990s in our country, some provinces and cities carried out experimental demonstrations of fermentation bed culture technology, but they have not been further popularized and applied. In recent years, Gansu Province has gradually introduced and vigorously promoted this technology, but due to the lack of suitable microbial agents The product and the matching technology cause the cost of raising pigs in the fermentation bed to be too high and the production is unstable, so it is difficult to promote in practical application
[0004] Gansu Province is located in a h

Method used

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  • Complex microbial inoculum for swine production by fermentation bed started quickly at low temperature and preparation method thereof
  • Complex microbial inoculum for swine production by fermentation bed started quickly at low temperature and preparation method thereof
  • Complex microbial inoculum for swine production by fermentation bed started quickly at low temperature and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Bacillus subtilis, Trichoderma spp, Rps.globiformis, Lactobacillus acidophilus, Saccharomyces cerevisiae and Streptomyces ) composed of 6 strains in total.

[0032] Bacterial agent preparation method: the above-mentioned 6 strains of functional microorganisms are inserted into the prepared six seed culture media according to the inoculation amount of 5% by volume percentage and cultivated respectively. The aerobic bacteria were sealed with three layers of thick sealing film, and cultured in a constant temperature incubator at 35°C for 72h, and the rest of the aerobic bacteria were sealed with two layers of 10μm gas-permeable membranes, and cultured in a constant temperature shaking culture bed at 32°C for 48h-72h. During the period, the number of viable bacteria in each bacterial culture solution was detected separately, and the number of viable bacteria in each bacterial culture was ≥ 1×10 8 After each / ml, take out seed liquid, by volume ratio is Trichoderma: Bacillus...

Embodiment 2

[0041] Repeat embodiment 1, following difference is arranged: by Trichoderma: Bacillus subtilis: Rhodopseudomonas sphaeroides: Lactobacillus acidophilus: Saccharomyces: Streptomyces=15%: 20%: 20%: 15%: 15% : 15%, measured under the same conditions in Example 1, see Table 1 for details.

[0042] The measurement results:

[0043] Pigs began to enter on the 8th day, and the continuous pig production in the normal fermentation bed was carried out. See Table 2 for details.

Embodiment 3

[0045] Repeat embodiment 1, have following difference: by Trichoderma: Bacillus subtilis: Rhodopseudomonas sphaericus: Lactobacillus acidophilus: Saccharomyces: Streptomyces=18%: 18%: 19%: 15%: 15% : 15%, measured under the same conditions in Example 1, see Table 1 for details.

[0046] Measuring results: the pigs were fed on the 6th day, and the continuous pig production in the normal fermentation bed was carried out, see Table 2 for details.

[0047] In order to prove the efficacy of the embodiments of the present invention, comparative examples are specially set up for testing.

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Abstract

The invention discloses a complex microbial inoculum for swine production by a fermentation bed started quickly at low temperature and a preparation method thereof. The complex microbial inoculum comprises the following microorganisms: 15-20% of trichoderma, 15-20% of bacillus subtilis, 15-20% of rhodopseudomonas spheroids, 15% of lactobacillus acidophilus, 15% of yeast and 15% of streptomyces avermitilis. The preparation method of the complex microbial inoculum comprises the steps of inoculating and propagating the microorganisms till each microorganism contains 108 viable bacteria per ml; mixing the microorganisms together according to the proportion thereof; and inoculating the mixed microorganisms in a low-cost culture medium for culturing to obtain the complex microbial inoculum. When used for fermenting fillings in a fermentation bed, the complex microbial inoculum has the advantages of fast temperature rise from low temperature of 0 DEG, strong deodorization function, short fermentation cycle and the like. The production cost of the complex microbial inoculum is 20% lower than that of the similar product. In addition, the complex microbial inoculum realizes continuous loop fermentation process of the fermentation bed in fermentation bed swine production, effectively solves the problem that livestock matures of large-scale culturing farm pollutes the environment, and also realizes the comprehensive development and utilization of waste resources.

Description

technical field [0001] The invention relates to the field of preparation of microbial inoculum, in particular to a low-temperature quick-start pig-raising composite microbial inoculum and a preparation method thereof. Background technique [0002] Fermentation bed pig raising technology is a theory that integrates biological fermentation, ecological cycle, health immunity, environmental control, and animal husbandry production. It is based on the establishment of a micro-ecosystem fermentation bed consistent with the local environment. It is a new eco-environmental protection pig raising technology that is characterized by zero emission and aims at producing safe livestock products. This technology first started in Japan in the 1970s, and then Europe, America and other countries successively carried out research and promoted it. [0003] In the 1990s in our country, some provinces and cities carried out experimental demonstrations of fermentation bed culture technology, but...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/16C12N1/20A01K1/015C12R1/885C12R1/125C12R1/38C12R1/23C12R1/645C12R1/465
Inventor 周剑平王治业
Owner 甘肃绿能农业科技股份有限公司
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