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Fungal inoculant for controlling bacterial wilt of tomato and preparation method thereof

A technology for tomato bacterial wilt disease and fungal inoculum is applied in the field of fungal inoculum for preventing and controlling tomato bacterial wilt and its preparation field, which can solve the problems such as the limited adaptability range of bacterial strains, achieve low production cost, reduce environmental pollution, and reduce sources of rich effects

Inactive Publication Date: 2011-10-19
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are already some microbial agents and bacterial fertilizers on the market that use pathogen antagonistic bacteria to control plant diseases, and there are also some reports on the biological control of tomato bacterial wilt, but there is no report on a product that has a good field application effect and stable control effect.
Moreover, the effect of using microbial agents and fertilizers is greatly affected by environmental conditions, and strains from different environmental conditions have a limited range of adaptation

Method used

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  • Fungal inoculant for controlling bacterial wilt of tomato and preparation method thereof
  • Fungal inoculant for controlling bacterial wilt of tomato and preparation method thereof
  • Fungal inoculant for controlling bacterial wilt of tomato and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: (Isolation and purification of fungi)

[0054] 1 medium

[0055] Martin's (Martin) medium: KH 2 PO 4 1.0g, glucose 10.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5.0g, agar 15-20g, water 1000ml; 1000ml of this culture medium plus 3.3ml of 1% red bengal aqueous solution; sterilization at 112.6°C for 30 minutes; add 1% streptomycin solution to every 100ml medium before use 0.3ml (30ppm);

[0056] 2 test steps

[0057] 2.1 Medium preparation: prepare Martin's medium to prepare fungal slope;

[0058] 2.2 Preparation of soil dilution

[0059] (1) Weigh 10g of soil, add 100ml sterile water with glass beads to a 500ml Erlenmeyer flask, shake for 15 minutes, that is 10 -1 ;

[0060] (2) Draw 10ml of soil suspension, add 90ml of sterile water to a 500ml Erlenmeyer flask, namely 10ml -2 ;

[0061] (3) Draw 10ml from (2), add 90ml sterile water to a 500ml Erlenmeyer flask, which is 10 -3 ;

[0062] 2.3 Fungus isolation

[0063] (4) Separate 0.1ml from (1)(2)(3), add to Martin's medium plate for...

Embodiment 2

[0068] Example 2: (Plate antagonistic test of isolated fungi against Ralstonia solanacearum on tomato bacterial wilt disease)

[0069] 1. Medium

[0070] 1.1 Solid medium:

[0071] Ralstonia solanacearum culture medium preparation: MgSO 4 7H 2 O0.3g, K 2 HPO 4 2.0g, 4.0g yeast extract, 8.0g sucrose hydrolysate, 10.0g sucrose, 18g agar, 1000ml distilled water;

[0072] Martin's medium preparation: same as in Example 1 2.1

[0073] 1.2 Liquid culture medium:

[0074] Tomato Ralstonia solanacearum liquid shake flask culture medium; Ralstonia solanacearum culture medium does not add agar;

[0075] 2. Test procedure

[0076] 2.1 Medium preparation:

[0077] Prepare Ralstonia solanacearum culture medium (solid, liquid), prepare the pathogen Ralstonia solanacearum slant;

[0078] Prepare Martin's medium (solid) to prepare fungal slopes;

[0079] 2.2 Plate Antagonism Test

[0080] 2.2.1 Strain activation

[0081] The tomato bacterial wilt bacteria and fungi (isolated bacteria in Example 1) are activate...

Embodiment 3

[0087] Example 3: (Preparation 1 of strain 6-18 fermentation solid inoculum)

[0088] Follow the steps below:

[0089] (1) Preparation of various levels of culture medium, including:

[0090] a) Slant medium: Martin's solid medium (same as in Example 1), spare;

[0091] b) Shake flask culture medium: potato sucrose liquid medium, spare;

[0092] c) Production fermenter culture medium: same as shake flask culture medium, spare;

[0093] Potato sucrose liquid medium: 20% potato extract 1000ml + sucrose 20g; among them, the preparation of 20% potato extract: weigh 200g of peeled potato pieces, add 1000ml of water and cook until the potato pieces can be pierced by the glass rod, filter, and make up the original Water volume.

[0094] (2) Inclined strain culture: Inoculate CGMCC No.3335 strain 6-18 on step (1) Inclined surface medium, cultivate in an incubator at 28-32℃ for 3-4 days, take it out and use it directly or put it in Refrigerate and store in a refrigerator at 4-7℃;

[0095] (3) Pro...

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Abstract

The invention discloses fungal inoculant for controlling bacterial wilt of tomato and a preparation method thereof, which belongs to the technical field of biological control of vegetable disease. The trichoderma gamsii strain 6-18 No. 3335 in the CGMCC is used as the strain, the Martin's solid medium is used as the slant medium, the potato sucrose liquid medium is used as the shaking flask strain medium and fermenter medium, and the liquid or solid fungal inoculant is prepared through multi-stage fermentation. The trichoderma gamsii strain is firstly used as the strain and prepared into the fungal inoculant through fermentation, thereby providing a new effective path for the biological control of bacterial wilt of tomato; the fungal inoculant achieves the disease control effect of more than 50%, thereby reducing the use amount of pesticides, and reducing the pollution to environment; the raw material of the fungal inoculant is rich, the fermentation process is simple, and the production cost is low, thus the fungal inoculant can be popularized and applied to the tomato planting areas and pesticide manufacture enterprises.

Description

Technical field [0001] The invention relates to the technical field of biological control of vegetable diseases, in particular to a fungal agent for preventing and curing tomato bacterial wilt and a preparation method thereof. Background technique [0002] Tomato bacterial wilt is caused by Ralstonia solanacearum (smith) Yabuuchi et al. It is a devastating soil-borne disease that spreads widely around the world. Tropical and subtropical countries and regions, such as: Serious occurrences occurred in the Americas, Japan, India, the Philippines, Taiwan, Guangdong, Zhejiang, Fujian, Jiangsu, Hubei, Sichuan, and Chongqing. The disease can cause large-scale wilting and death of plants. The incidence rate of general fields is 10%-15%, and the incidence rate of severely ill fields is as high as 80%-98.5%, which leads to a serious decline in tomato production and major economic losses. [0003] R. solanacearum is a soil-habiting bacteria that can survive for a long time in soil without a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00C12R1/885
Inventor 薛智勇朱凤香王卫平洪春来陈晓旸吴传珍杨友坤奚辉
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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