Fungus for promoting orchid seeds to germinate

A technology of plant seeds and fungi, applied in the field of bioengineering, can solve the problems of limited popularization and application, no large-scale cultivation base, lack of systematic research, etc., and achieve the effect of huge application value

Inactive Publication Date: 2010-12-15
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical application, when the aseptic seedlings obtained by tissue culture rapid propagation are used for cultivation, the two links of training seedlings and transplanting not only limit the popularization and application of this method in terms of technology but also production costs.
Before a breakthrough in technology in this area, it is still difficult to achieve large-scale cultivation and production of Dendrobium plants.
[0007] There are only scattered reports about the cultivation techniques of Bletilla striata, but there is a lack of systematic research, and there is no large-scale cultivation base. There are still many problems to be solved in the traditional method of asexual reproduction of wild plants.
Wild mutated species need a long domestication process, but so far there a

Method used

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  • Fungus for promoting orchid seeds to germinate
  • Fungus for promoting orchid seeds to germinate
  • Fungus for promoting orchid seeds to germinate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation of germination fungi

[0023] Seeds are sown in a suitable growth environment for bletilla striata and dendrobium to obtain seeds, protocorms and seedlings, and germination fungi are obtained by separating and screening after surface disinfection of germination materials.

Embodiment 2

[0025] Effect of Bsg14 on germination of Dendrobium officinale seeds

[0026] Sow the seeds of Dendrobium candidum on the wheat bran, leaves and sawdust that have been covered with Bsg14 fungal mycelium, and use the sterilized wheat bran, leaves and sawdust as a control, and cultivate them in a culture room at a temperature of 23-27 ℃, 12 hours of light per day, the light intensity is: 1200Lx, the seed germination situation is investigated in two months, the result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 67.3%, and the seed germination of Dendrobium candidum treated without fungus The rate is 0.0%.

Embodiment 3

[0028] Effect of Bsg14 on germination of Dendrobium officinale seeds

[0029] Sow the seeds of Dendrobium candidum on the wheat bran, leaves and sawdust that have been covered with Bsg14 fungal mycelia, and use the sterilized wheat bran, leaves and sawdust as a control, and cultivate them in a greenhouse at a temperature of 15-26 °C , natural light, the daily light time is 8-10 hours, the seed germination situation is investigated in two months, the result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 65.1%, and the seed germination of Dendrobium candidum without fungus treatment The rate is 0.0%.

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Abstract

The invention discloses a fungus for promoting orchid seeds to germinate. The classified name of the fungus is tulasnellasp. fungus Bsg14 with the preservation number of CGMCC No.2451. The strain has important significance for solving the seed germination problems of dendrobium plants in orchidaceae and bletilla plants, can be sown with the orchid seeds and capable of used for culturing seedling-raising trays, flowerpots or seedbeds or cultivating the land for growing field crops and has application value for obtaining the seedlings instead of tissue culture.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to three fungal strains for promoting the germination of orchid seeds, which can be sown together with the orchid seeds and used for seedling cultivation in seedling trays, flower pots or seedbeds or field cultivation. Background technique [0002] There are many traditional precious Chinese medicines in orchid plants, such as dendrobium and bletilla striata. According to the records of the 2005 edition of the "Chinese Pharmacopoeia", Dendrobium has the effects of nourishing the stomach and promoting fluid, nourishing yin and clearing heat, and can also relieve throat and improve eyesight, and regulate human immune function. Bletilla striata is used as a tuber, which has the effects of astringent and hemostasis, detumescence and muscle growth. It is mainly used to treat hemoptysis and hematemesis, traumatic bleeding, sore swelling, chapped skin, tuberculosis hemoptysis, and u...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/00C12R1/645
Inventor 郭顺星房慧勇陈晓梅肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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