Fungus for promoting germination of bletilla striata seeds

A technology of seeds and Bletilla striata, applied in the field of bioengineering, can solve the problems of limited popularization and application, lack of systematic research, and lack of large-scale cultivation bases, and achieves the effect of huge application value.

Inactive Publication Date: 2010-12-15
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical application, when the aseptic seedlings obtained by tissue culture rapid propagation are used for cultivation, the two links of training seedlings and transplanting not only limit the popularization and application of this method in terms of technology but also production costs.
Before a breakthrough in technology in this area, it is still difficult to achieve large-scale cultivation and production of Dendrobium plants.
[0007] There are only scattered reports about the cultivation techniques of Bletilla striata, but there is a lack of systematic research, and there is no large-scale cultivation base. There are still many problems to be solved in the traditional method of asexual reproduction of wild plants.
Wild mutated species need a long domestication process, but so far there are no reports on this aspect
It is an effective way to use tissue culture to solve the problem of reproduction and germplasm preservation of endangered plants. At present, some units have carried out research on tissue culture and rapid propagation of seeds, shoot tips and lateral buds, and pseudobulbs. Aseptic seedlings can be obtained within the country, but seedling training and transplanting are a problem that limits its application. At present, there is no effective method for training seedlings and transplanting. Only after a breakthrough in this technology can it be practically applied
[0009] At present, there are few domestic and foreign research reports on fungi that promote seed germination of orchid medicinal plants, and no excellent fungi that can promote seed germination have been found.

Method used

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  • Fungus for promoting germination of bletilla striata seeds
  • Fungus for promoting germination of bletilla striata seeds
  • Fungus for promoting germination of bletilla striata seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation of germination fungi

[0023] Seeds are sown in a suitable growth environment for bletilla striata and dendrobium to obtain seeds, protocorms and seedlings, and germination fungi are obtained by separating and screening after surface disinfection of germination materials.

Embodiment 2

[0025] Effect of Bsg14 on germination of Dendrobium officinale seeds

[0026] Sow the seeds of Dendrobium candidum on the wheat bran, leaves and sawdust that have been covered with Bsg14 fungal mycelium, and use the sterilized wheat bran, leaves and sawdust as a control, and cultivate them in a culture room at a temperature of 23-27 ℃, 12 hours of light per day, the light intensity is: 1200Lx, the seed germination situation is investigated in two months, the result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 67.3%, and the seed germination of Dendrobium candidum treated without fungus The rate is 0.0%.

Embodiment 3

[0028] Effect of Bsg14 on germination of Dendrobium officinale seeds

[0029] Sow the seeds of Dendrobium candidum on the wheat bran, leaves and sawdust that have been covered with Bsg14 fungal mycelia, and use the sterilized wheat bran, leaves and sawdust as a control, and cultivate them in a greenhouse at a temperature of 15-26 °C , natural light, and the light time per eye is 8-10 hours, and the seed germination situation is investigated in two months. The result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 65.1%, and the seed germination of Dendrobium candidum without fungus treatment The rate is 0.0%.

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Abstract

The invention discloses a fungus for promoting the germination of bletilla striata seeds. The fungus is named as Bsg11 and classified as Rhizoctonia sp. and has the preservation number of No.2450 in the CGMCC (China General Microbiological Culture Collection Center). The strain has an important significance on solving the problem of germination of the bletilla striata seeds, can be sown together with the bletilla striata seeds, is used for culturing seedlings by seedling-culturing pans or flowerpots or seedbeds and cultivating field crops on land and has the application value of replacing tissue culture to obtain plant seeds.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to three fungal strains for promoting the germination of orchid seeds, which can be sown together with the orchid seeds and used for seedling cultivation in seedling trays, flower pots or seedbeds or field cultivation. Background technique [0002] There are many traditional precious Chinese medicines in orchid plants, such as dendrobium and bletilla striata. According to the records of the 2005 edition of the "Chinese Pharmacopoeia", Dendrobium has the effects of nourishing the stomach and promoting fluid, nourishing yin and clearing heat, and can also relieve throat and improve eyesight, and regulate human immune function. Bletilla striata is used as a tuber, which has the effects of astringent and hemostasis, detumescence and muscle growth. It is mainly used to treat hemoptysis and hematemesis, traumatic bleeding, sore swelling, chapped skin, tuberculosis hemoptysis, and u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/00C12R1/645
Inventor 郭顺星房慧勇陈晓梅肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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