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Compositions and methods for making androstenediones

A plant and steroid technology, applied in the directions of botanical equipment and methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as high cost and limited economic utility

Inactive Publication Date: 2010-12-15
VERENIUM CORP (US)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In an earlier pilot-scale trial of AD production using Mycobacterium sp. B3683 (Marshek (1972) supra), the production of large amounts of ADD and compounds X1 and X2 limited The economic utility of this approach

Method used

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  • Compositions and methods for making androstenediones
  • Compositions and methods for making androstenediones
  • Compositions and methods for making androstenediones

Examples

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Embodiment 1

[0405] Example 1: Preparation and use of exemplary genes and host cells of the invention

[0406] This example describes the preparation and use of exemplary host cells of the invention to produce 1,4-androsdiene-3,17-dione (ADD) and related pathway compounds, including 20-(hydroxymethyl)pregna- 4-en-3-one and 20-(hydroxymethyl)pregna-1,4-dien-3-one.

[0407] In one aspect, the modified host cells of the invention provided by the invention are bacterial cells, such as Mycobacterium strains, such as those designated B3683 (see, e.g., Pérez et al. (1995) Biotechnology Letters 17(11):1241-1246) and B3805 (See e.g., (1998) Acta Microbiol Pol. 47(4):335-343) Mycobacterium strains. Mycobacterium sp. B3683 was produced by mutagenesis from soil isolates to eliminate complete degradation of phytosterols and to be able to produce ADD and AD. Because the B3683 strain produced significantly more ADD than AD, Mycobacterium sp. B3805 was derived from B3683 by mutagenesis to reduce ADD p...

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Abstract

The invention provides compositions and methods for producing androstenedione (4-androstenedione), of improved purity and for modulating its production, for example by deletion or inactivation of ksdA, cxgA, cxgB, cxgC, or cxgD. The invention also provides methods and compositions, including nucleic acids that encode enzymes, for producing 1,4-androstadiene-3,17-dione (ADD) and related pathway compounds, including 20-(hydroxymethyl) pregna-4-en-3-one and 20-(hydroxymethyl)pregna-1,4-dien-3-one. The compositions of the invention include nucleic acids, probes, vectors, cells, transgenic plants and seeds, transgenic animals, kits and arrays.

Description

[0001] Refer to the sequence listing submitted via EFS-WEB [0002] The entire content of the electronic submission of the following Sequence Listing is hereby incorporated by reference in its entirety for all purposes through the USPTO EFS-WEB server authorized and filed under MPEP § 1730 II.B.2(a)(C). The Sequence Listing is identified on the following electronically filed text file: [0003] file name date of formation size (bytes) 564462016440Seqlist.txt November 13, 2008 120,834 bytes field of invention [0004] The present invention relates generally to biology and medicine. The present invention provides for the production of androstenedione (AD, or 4-androsten-3,17-dione) of increased purity and modulation thereof, for example, by deletion or inactivation of the ksdA, cxgA, cxgB, cxgC or cxgD genes or gene activity. generated method. The invention also provides for the production of 1,4-androsdiene-3,17-dione (ADD) and related ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/35C07K16/12C12N1/15C12N1/19C12N1/21C12N5/10C12N5/20C12N9/00C12N9/02C12N9/10C12N15/31C12N15/52C12N15/53C12N15/54G01N33/50
CPCC12P33/00C07K2319/20G01N2500/04C07K2319/40C12N9/1029G01N33/743C07K14/35C12N9/001
Inventor D·南恩C·普约奥K·查特曼
Owner VERENIUM CORP (US)
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