Breeding method of rice variety with stripe vein marked characters

A technology of leaf veins and rice, applied in the field of breeding of rice varieties marked with striped veins, can solve the problems of difficult definition, limitation, and poor effect, and achieve the effects of increasing diversity, preventing counterfeiting, and ensuring safety

Inactive Publication Date: 2011-01-19
WIN ALL HI TECH SEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the past, the traditional identification method was to identify a specific variety based on the morphological characteristics of the plant and the botanical classification method. For some varieties with no obvious differences between varieties, it was difficult to define and the effect was poor.
The DUS measurement technology currently used in the national variety protection is a very effective protection method, but due to the high content of identification technology, the units that can engage in technical identification are limited, the cost is high, and the application is limited.
[0004] There are known methods for marking rice leaf appearance traits, mainly using the difference in leaf color during the vegetative growth period of rice, such as using light green leaf traits, utilizing purple leaf traits, and using green-turning albino mutations to mark rice plants. Most of these leaf color ma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] With a radiation dose of 3000N + The seeds of the rice variety "Wuyujing 7" were treated with ion beam physical mutagenesis, and the mutagenized species M 0 , planting M 0 There are 3680 plants in the population, and one copy of the mutant with white striped vein marks is obtained, and the material is collected separately and numbered "Biao 2005"; this "Biao 2005" M is planted separately 1 There are 1426 single-line populations, and the central veins of the plants at the seedling stage all have white stripes to mark the traits. After the selection of individual plants and mixed harvesting, continuous planting selection until M 4 In the same generation, the performance of the plant population was consistent, and the trait of white striped veins was genetically expressed stably. This material was named "Quanjingbiao 2005".

[0018] F 1 . Plant F 1 , observe F 1 The color of the veins of the plant is green; use this F 1 The plant pollen was backcrossed with "Quanjin...

Embodiment 2

[0020] Planting hybrid backcross BC of "Quanjingbiao 2005" and indica rice "Yangdao 6" 1 f 1 Population, select dominant plants with white stripe leaf vein traits and continue planting to segregate and select to BC 1 f 3 , will bc 1 f 3 The plant was backcrossed again with the indica rice "Yangdao 6" to obtain BC 2 f 1 Seeds were planted and segregated to select plants with traits similar to "Yangdao No. 6" with white stripe vein trait markers, and continuous self-selection was carried out until BC 2 f 6 , the plant traits have been stable and consistent, named "Quanbiao Xian 1".

[0021] "Quanjingbiao 2005" with white striped veins was crossed with blast-resistant wide-compatibility material MP3. The breeding method was the same as that of "Quanbiao Xian 1", and the molecular marker-assisted selection technique was used to breed and produce The blast-resistant japonica rice variety "Quanbiaojing 1" with white striped veins.

[0022] "Quanjingbiao 2005" with white str...

Embodiment 3

[0024] Treat the seeds of the rice variety "YR1671" with 0.20 mol / l of ethyl methanesulfonate (EMS) for 50 minutes to obtain the mutagenic treatment variety M 0 , planting M 0 A population of 4682 plants obtained a mutant material marked with yellow-striped veins, which was collected individually and numbered "Biaoxuan 01"; this "Biaoxuan 01" was planted separately. 1 There are 925 single-line populations, and 634 plants in the center of the seedling veins show yellow stripes as marking traits. After optimization of individual plants and mixed harvesting, continuous planting selection to M 4 In the next generation, the performance of the plant population was consistent, and the trait of yellow stripe veins was genetically expressed stably. This material was named "Quanbiao R01".

[0025] F 1 . Plant F 1 , observe F 1 The color of the veins of the plant is green; use this F 1 The plant pollen was backcrossed to "Quanbiao R01", and the backcross variety BC was obtained 1 ...

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Abstract

The invention relates to a breeding method of a rice variety with stripe vein marked characters, which is characterized by comprising the following steps: carrying out physicochemical mutation treatment by utilizing ion beams or gamma rays and ethylmethane sulfonate, inducing color variation of rice vein stripes, and screening rice vein mutants; selecting the mutant marked with vein stripes on blades for individually planting until the mutant is stably expressed; hybridizing and backcrossing the stripe vein mutation material with the conventional rice, analyzing the genetic rules of the character, hybridizing and backcrossing the stripe vein mutation material with the conventional rice and the parents of the hybrid rice, identifying the fertility, carrying out test-crossing and mating tests, and selecting two nuclear sterile lines marked with recessive stripe veins, a restoring line, three maintenance lines and a conventional rice material; and matching the two bred nuclear sterile lines, the bred restoring line and the bred maintenance lines, and verifying the validity to acquire hybrid rice parents, hybrid rice combination and conventional rice varieties marked with veins. The invention realizes the purposes of quickly, simply, conveniently, visually and accurately identifying the characteristics and the individualities of rice varieties and quickly and accurately identifying the purity of varieties in the seedling stage, and the invention increases new gene types of rice.

Description

technical field [0001] The invention relates to a method for breeding rice varieties marked with striped veins. Background technique [0002] Rice plays an important role in my country's grain production, and the production of rice seeds is the top priority of my country's food security. With the promulgation of the "Seed Law" and the rapid advancement of seed industrialization, the rice seed industry has received more and more attention from all walks of life. The continuous breeding of high-yield and high-quality new rice varieties has made a significant contribution to the increase in grain production and income in my country. However, incidents of counterfeit and shoddy seeds pitting farmers also continue to occur. On the one hand, the main reason is biological mixing (such as string powder) in the seed production process, parental mixing and mechanical mixing in the process of seed harvesting, processing, storage and transportation; Due to intellectual property rights,...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H1/06
Inventor 张从合刘斌美陈金节吴跃进蒋家月严志陈琳周桂香刘兴江张云虎申广勒陶亮之
Owner WIN ALL HI TECH SEED CO LTD
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