Culture medium capable of improving viable count of lactic acid bacillus

Abstract

The invention discloses a culture medium capable of improving viable count of lactic acid bacillus, which comprises 0.003 to 0.006 percent of biotin, 0.01 to 0.05 percent of vitamin E, 0.01 to 0.03 percent of tween 800, 1 percent of yeast extracts, 1.5 to 2.0 percent of glucose, 0.15 to 0.2 percent of dipotassium phosphate, 0.08 to 0.15 percent of magnesium sulfate, 0.03 to 0.06 percent of manganese sulfate and the balance of water according to the total mass fraction of 100 percent. The culture medium capable of improving the viable count of the lactic acid bacillus is taken as a culture medium of the lactic acid bacillus, the lactic acid bacillus is cultured according to the conventional culture method, the viable count of final fermentation liquor can reach over 1011 CFU / mL, so that the culture medium has good culture effect, can be used for the proliferation of the lactic acid bacillus, and has important meaning for industrialized production.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and in particular relates to a culture medium capable of increasing the number of live bacteria of lactic acid bacillus. Background technique: [0002] Since the 1950s, antibiotics have been widely used in feed as growth promoters. However, due to the long-term use of antibiotics, the normal intestinal flora of animals is out of balance, resulting in drug-resistant strains, and the residues of antibiotics in livestock and poultry products directly affect human health and safety and the ecological environment. Many countries and breeders oppose antibiotics as growth promoters, and many countries such as the European Union and the United States have restricted the use of antibiotics in legislation. In my country, due to the food safety incidents that have occurred in recent years, people's health awareness has been continuously enhanced and they are increasingly enthusiastic about green and heal...

AI Technical Summary

Problems solved by technology

[0004] Among the probiotic preparations, lactic acid bacteria preparations are the most concerned. Lactic acid bacteria are one of the dominant flora in the intestinal tract of animals. They can produce acids and bacteriocins, inhibit the growth and reproduction of harmful bacteria, maintain the balance of intestinal flora, and produce Immune activity, and the effect of lactic acid bacteria that can enter the intestinal tract in living bodies is much greater than that of dead bacteria, but generally lactic acid bacteria have higher nutritional requirements and poor resistance to adverse environments, especially the ability to resist dryness and high temperature. Poor performance, not conducive to feed processing, transportation and storage

As an active factor, the ability to resist gastric acid and successfully reach the intestinal tract and survive colonization is weak