Thermoduric bacteria composite culture medium and thermoduric bacteria detection method
A technology for heat-resistant bacteria and culture medium, which is applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., can solve problems such as inaccurate detection results of heat-resistant bacteria, and achieve outstanding substantive characteristics, prominent colony characteristics, and easy effect of counting
Active Publication Date: 2013-07-03
烟台喜力科技发展有限公司
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AI Technical Summary
Problems solved by technology
[0005] The invention mainly solves the problem that the existing culture medium is used to detect heat-resistant bacteria in solid food and the detection result is inaccurate
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0022] Example 1, accurately weigh 1.5g of tryptone, 6.0g of bacterial agar powder, 0.6g of beef extract, 0.9g of yeast extract, 0.4g of glucose, 0.04g of bromocresol purple, 0.08g of complex vitamin B, and calcium nitrate: 0.08 g, Tween 80 0.4g, after mixing, it is a thermostable bacteria culture medium.
Embodiment 2
[0023] Example 2, accurately weigh 1.2g of tryptone, 6.5g of bacterial agar powder, 0.4g of beef extract, 0.8g of yeast extract, 0.5g of glucose, 0.05g of bromocresol purple, 0.06g of complex vitamin B, calcium nitrate: 0.09 g, Tween 80 0.4g, after mixing, it is a thermostable bacteria culture medium.
Embodiment 3
[0024] Example 3, accurately weigh 1.7g of tryptone, 5.5g of bacterial agar powder, 0.7g of beef extract, 1g of yeast extract, 0.44g of glucose, 0.01g of bromocresol purple, 0.09g of complex vitamin B, and 0.06g of calcium nitrate , Tween 80 0.5g, after mixing, it is a medium for heat-resistant bacteria.
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The invention discloses a thermoduric bacteria composite culture medium which is characterized by comprising the following materials in percentage by weight: 10-20% of tryptone, 50-70% of bacterial agar powder, 1-10% of beef extract, 1-10% of yeast extract, 1-10% glucose and 0.1-0.9% of bromocresol purple. The thermoduric bacteria detection method comprises the following steps: adding aseptic diluent to a sample, homogenizing, using the homogenized diluent for an experiment, inoculating the diluent, pouring the thermoduric bacteria composite culture medium, culturing in an incubator at 36+ / -1DEG C for 48+ / -2h, and counting to obtain the thermoduric bacteria detection result. By using the thermoduric bacteria composite culture medium, the quantity of residual sporeformers in solid food atthe central temperature of thermal treatment can be accurately detected, the detection rate is 20% higher than that of the market culture medium, and the colony has obvious characteristics which facilitate the counting procedure.
Description
Technical field: [0001] The invention relates to the technical field of detection of heat-resistant bacteria in solid food, in particular to a compound culture medium for heat-resistant bacteria and a detection method for heat-resistant bacteria. Background technique: [0002] Thermostable bacteria is a general term for a class of bacteria that contain spores and survive heat treatment. Under suitable conditions, the spores can be activated into nutrients and cause food spoilage. At present, the research on thermostable bacteria mainly involves acidophilic thermostable bacteria in fruit juices. etc., its detection methods and reagents are all aimed at liquid food, not suitable for the detection of heat-resistant bacteria in solid food; the existing standard SN 0178 "Counting of thermophilic spores (total number of aerobic spores, flat acid spores and anaerobic spores) in exported food "Method" used glucose tryptone agar, but the detection rate of thermostable bacteria on glu...
Claims
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IPC IPC(8): C12Q1/06
Inventor 徐世明赵瑞连徐冬雪宋维娟
Owner 烟台喜力科技发展有限公司