Preparation method of deer placenta oligopeptides
A deer placenta and oligopeptide technology, which is applied in the fields of medicine and health care products, can solve the problems such as the inability to make full use of medicinal components, and achieve the effects of less allergic reaction, high yield and small molecular weight.
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Embodiment 1
[0014] For fresh deer placenta, wash the blood with clean water, remove connective tissue, blood vessels and fat, drain the surface of the placenta, cut into 1.0-1.3 cm in size 3 Fragments left and right. Add 3 times the mass of 0.9% saline to the shredded placenta for tissue grinding, and high-speed tissue homogenization at 12000r / min for 20 minutes. The homogenized placenta was put into a 200w power ultrasonic breaker and crushed 3 times, each time for 10 seconds, with an interval of 25 seconds between each time. The broken placenta was frozen at -18°C, thawed naturally at room temperature after 24 hours, and the operation was repeated twice. The frozen-thawed placenta was placed in a refrigerated centrifuge at 4° C., subjected to refrigerated centrifugation at 6000 r / min for 25 minutes, and the supernatant was freeze-dried to obtain a powder containing deer placenta oligopeptides. This is the best preparation method of deer placenta oligopeptides, and the content of deer...
Embodiment 2
[0016] For fresh deer placenta, wash the blood with clean water, remove connective tissue, blood vessels and fat, drain the surface of the placenta, cut into 1.0-1.3 cm in size 3 Fragments left and right. Add 3 times the mass of 0.7% saline to the shredded placenta for tissue grinding, and homogenize the tissue at a high speed of 10000r / min for 25 minutes. The homogenized placenta was put into a 200w power ultrasonic breaker and crushed 5 times, each time for 15 seconds, with an interval of 25 seconds between each time. The broken placenta was frozen at -18°C, thawed naturally at room temperature after 48 hours, and the operation was repeated 3 times. Put the frozen-thawed placenta into a refrigerated centrifuge at 4°C, perform refrigerated centrifugation at 6000r / min for 30 minutes, take the supernatant and freeze-dry to obtain a powder containing deer placenta oligopeptides. The content of deer placenta oligopeptides in the powder was 24.8%.
Embodiment 3
[0018] For fresh deer placenta, wash the blood with clean water, remove connective tissue, blood vessels and fat, drain the surface of the placenta, cut into 1.0-1.3 cm in size 3 Fragments left and right. Add 4 times the mass of 0.9% saline to the shredded placenta for tissue grinding, and homogenize the tissue at a high speed of 12000r / min for 20 minutes. The homogenized placenta was put into a 200w power ultrasonic breaker and crushed 7 times, each time for 10 seconds, with an interval of 25 seconds between each time. The broken placenta was frozen at -18°C, thawed naturally at room temperature after 36 hours, and the operation was repeated twice. Put the frozen-thawed placenta into a refrigerated centrifuge at 4°C, perform refrigerated centrifugation at 6000r / min for 35 minutes, take the supernatant and freeze-dry to obtain a powder containing deer placenta oligopeptides, the content of deer placenta oligopeptides in the powder was 26.4%.
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