The invention relates to a freeze-dried 
rabies vaccine for humans and a preparation method of the vaccine, relates to the field of 
vaccine production preparation technologies and aims at solving the problems that effective 
virus antigen expression content is low, the 
side effect rate of a vaccinator is high and vaccine yield and quality can not meet standard requirements as only a 
biological reactor is adopted for producing a 
rabies vaccine. The freeze-dried 
rabies vaccine for humans is obtained by inoculating aG strain 
rabies virus on Vero cells and sequentially carrying out 
ultrafiltration and concentration, separation and purification as well as 
freeze drying, wherein the packing volume of the freezed-dried 
rabies vaccine for 
human use is 0.5ml / 
dose, and during 
freeze drying, the adopted vaccine freeze-
drying protecting agent comprises the following ingredients: 60-90g / l of trehalos, 6-14g / l of 
sodium glutamate, 3-6g / l of 
urea, 2-3g / l of L-
arginine and 10g / l of 199 culture medium, and the vaccine freeze-
drying protecting agent does not contain 
gelatin, 
human serum albumin or 
dextran. The freeze-dried 
rabies vaccine for humans has the advantages that cost is low, operation is easy, 
pollution is hardly produced, vaccine quality and yield are greatly improved, the content of impurities in a vaccine is reduced, 
allergy reactions are hardly caused, and 
vaccine safety is greatly improved.