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Preparation method of deer placenta oligopeptides

A deer placenta and oligopeptide technology, applied in the field of health care products and medicine, can solve the problems of not being able to make full use of medicinal ingredients, and achieve the effects of less allergies, small molecular weight, and no pollution to the production environment

Inactive Publication Date: 2011-03-09
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are already deer placenta products in the domestic market, such as dried deer placenta. The disadvantage is that the medicinal ingredients in it cannot be fully utilized, and they can only play a simple nourishing role.
Foreign countries such as Russia, North Korea and Japan have also studied deer placenta, but their research mainly focuses on the physiological functions of deer placenta, and there is no report on the active substance oligopeptide in deer placenta.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] For fresh deer placenta, wash the blood with clean water, remove connective tissue, blood vessels and fat, drain the surface of the placenta, cut into 1.0-1.3 cm in size 3 Fragments left and right. Add 3 times the mass of 0.9% saline to the shredded placenta for tissue grinding, and high-speed tissue homogenization at 12000r / min for 20 minutes. The homogenized placenta was put into a 200w power ultrasonic breaker and crushed 3 times, each time for 10 seconds, with an interval of 25 seconds between each time. The broken placenta was frozen at -18°C, thawed naturally at room temperature after 24 hours, and the operation was repeated twice. The frozen-thawed placenta was placed in a refrigerated centrifuge at 4° C., subjected to refrigerated centrifugation at 6000 r / min for 25 minutes, and the supernatant was freeze-dried to obtain a powder containing deer placenta oligopeptides. This is the best preparation method of deer placenta oligopeptides, and the content of deer...

Embodiment 2

[0016] For fresh deer placenta, wash the blood with clean water, remove connective tissue, blood vessels and fat, drain the surface of the placenta, cut into 1.0-1.3 cm in size 3 Fragments left and right. Add 3 times the mass of 0.7% saline to the shredded placenta for tissue grinding, and homogenize the tissue at a high speed of 10000r / min for 25 minutes. The homogenized placenta was put into a 200w power ultrasonic breaker and crushed 5 times, each time for 15 seconds, with an interval of 25 seconds between each time. The broken placenta was frozen at -18°C, thawed naturally at room temperature after 48 hours, and the operation was repeated 3 times. Put the frozen-thawed placenta into a refrigerated centrifuge at 4°C, perform refrigerated centrifugation at 6000r / min for 30 minutes, take the supernatant and freeze-dry to obtain a powder containing deer placenta oligopeptides. The content of deer placenta oligopeptides in the powder was 24.8%.

Embodiment 3

[0018] For fresh deer placenta, wash the blood with clean water, remove connective tissue, blood vessels and fat, drain the surface of the placenta, cut into 1.0-1.3 cm in size 3 Fragments left and right. Add 4 times the mass of 0.9% saline to the shredded placenta for tissue grinding, and homogenize the tissue at a high speed of 12000r / min for 20 minutes. The homogenized placenta was put into a 200w power ultrasonic breaker and crushed 7 times, each time for 10 seconds, with an interval of 25 seconds between each time. The broken placenta was frozen at -18°C, thawed naturally at room temperature after 36 hours, and the operation was repeated twice. Put the frozen-thawed placenta into a refrigerated centrifuge at 4°C, perform refrigerated centrifugation at 6000r / min for 35 minutes, take the supernatant and freeze-dry to obtain a powder containing deer placenta oligopeptides, the content of deer placenta oligopeptides in the powder was 26.4%.

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Abstract

The invention relates to a preparation method of deer placenta oligopeptides, which comprises the following steps: taking a fresh deer placenta, cleaning bloodiness, removing connective tissues, blood vessels and fat, draining the surface moisture of the placenta, and cutting the placenta into chips of which the sizes are about 1.0-1.3cm<3>; adding the cut placenta into 0.9% saline water of which the quality is 3 times of the quality of the cut placenta for grinding tissues, and homogenizing the tissues for 20min at a high rotation speed of 12000r / min; putting the homogenized placenta into an ultrasonic crusher with the power of 200W for crushing for 3 times for 10s each time at an interval of 25s; freezing the crushed placenta at -18 DEG C, naturally unfreezing the crushed placenta at ordinary temperature after 24h, and repeatedly carrying out operation for 2 times; and putting the frozen and thawed placenta into a refrigerated centrifuge at 4 DEG C for carrying out refrigerated centrifugation for 25min at 6000r / min, taking the supernatant liquid for refrigeration and drying to obtain the powder containing deer placenta oligopeptides. The preparation method of deer placenta oligopeptides of the invention has the advantages of simple preparation method and high yield and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the fields of medicine and health products, and in particular relates to a preparation method of deer placenta oligopeptide. Background technique [0002] Mammalian placenta contains various active substances such as immunoglobulins, amino acids, peptides, cytokines, etc. The oligopeptides are considered to be the most effective ingredients in deer placenta due to their unique structure, properties and functions. Improve human immunity, anti-oxidation, and anti-aging physiological effects. At present, there is still a lack of systematic and in-depth research on the deep processing of deer placenta at home and abroad. There are already deer placenta products in the domestic market, such as dried deer placenta, but its disadvantage is that the medicinal ingredients in it cannot be fully utilized, and it can only play a simple nourishing role. Foreign countries such as Russia, North Korea and Japan have also studied deer placent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
Inventor 皮钰珍岳喜庆王忠霞武俊瑞赵春燕杜阿楠
Owner SHENYANG AGRI UNIV
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