Method for preparing prostate cancer-resisting mussel enzymolytic extract and application thereof
An anti-prostate cancer and mussel enzyme technology, applied in the direction of medical preparations containing active ingredients, drug combinations, anti-tumor drugs, etc., to achieve a good effect of inhibition of proliferation
Inactive Publication Date: 2012-09-05
ZHEJIANG OCEAN UNIV
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But so far there is no report about the effect of mussel enzymatic hydrolyzate on prostate cancer cells
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[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0022] 1 Protease activity assay method
[0023] Drawing of tyrosine standard curve: Accurately weigh 0.10009 tyrosine that has been dried to constant weight, gradually add 6ml of 1M HCl to dissolve, use 0.2M HCl to set the volume to 100ml, the concentration is 1000μg / ml, and then absorb the solution 10ml, dilute to 100ml with 0.2M HCI, then make 100μg / ml tyrosine solution. Dilute the tyrosine solution to different concentrations of 0-100μg / ml, take 6 test tubes, draw 1ml of tyrosine with different concentrations, and add 0.4M Na 2 CO 3 5ml, and then add 1ml of diluted Folin reagent. Shake well and place in a water bath at 40°C for 20 minutes to develop color, and use a spectrophotometer to measure the optical density D0 value at 680nm. Take the net D0 value as the ordinate, and the concentration of tyrosine as the abscissa, and draw a sta...
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The invention relates to a method for preparing a prostate cancer-resisting mussel enzymolytic extract. The method comprises the following steps: 1) preparing a sample by cleaning mussel, removing the shell thereof, taking out the flesh and homogenating, wherein the ratio of solid to liquid is 1:2 to 1:3, the pH value is adjusted to 9.5 to 10 and the temperature is kept between 25 and 30 DEG C for 20 to 30 min; and 2) adding Alcalase, wherein the enzyme adding quantity is 1500 to 2000u / g, the enzymolysis temperature is 45 to 50 DEG C and the enzymolysis time is 6 to 8 h; and carrying out inactivation and centrifugation and taking the liquid supernatant so as to acquire the enzymolytic product. The invention also discloses an application of the enzymolytic extract in resisting the prostatecancer. Compared with the prior art, the invention has the advantages that a better technique is acquired by controlling the enzyme selection, the ratio of solid to liquid, the pH value, the adding quantity of enzyme, the temperature and the enzymolysis time, and the product has a good function of inhibiting the multiplication of prostate cancer cells.
Description
technical field [0001] The invention relates to a preparation method of mussel enzymolysis extract, which can be used to resist prostate cancer. Background technique [0002] Polypeptides can be used as active substances such as neurotransmitters, interleukins, and cell growth factors in organisms, and have active functions such as maintaining the growth and development of organisms, immune regulation, and metabolism. Existing studies have shown that peptides derived from organisms have anti-tumor, anti-oxidation, anti-bacterial, anti-aging, anti-hypertensive and other active functions, especially the anti-tumor activity has attracted the attention of researchers from various countries. At present, there have been a large number of reports on the anti-tumor activity of natural peptides and synthetic peptides, and some peptides have been used clinically as anti-tumor drugs; there are many studies on the anti-bacterial, antioxidant, and anti-hypertensive activities of peptides...
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IPC IPC(8): A61K38/01A61P13/08A61P35/00A61K35/56A61K35/618
Inventor 丁国芳杨永芳杨最素郁迪黄芳芳
Owner ZHEJIANG OCEAN UNIV