Method for producing potato virus A (PVA)-free seedling by utilizing variable temperature detoxification technology
A technology for potatoes and virus-free seedlings, which is applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as unfavorable mass production, poor adaptability, and long period of virus-free seedlings, and saves energy. , the effect of high efficiency and simple technical procedures
Inactive Publication Date: 2012-09-05
HUNAN AGRICULTURAL UNIV
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Problems solved by technology
The deficiencies of the above potato detoxification techniques are: first, the stem tip detoxification technology requires a high technical level and skilled skills; second, different potato varieties or strains require special formulas, and the adaptability is not wide; The cycle of seedlings is long, and the technology of virus-free seedlings obtained at one time is small, which is not conducive to short-term mass production
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Embodiment 1
[0022] 1) Transformation of tissue cultured seedlings: take the tubers of Zhongzhai Huangpi, a potato variety stored at normal temperature, and treat them under a dark condition of 25°C for 7 days to obtain shoots, and take the shoots and disinfect them with 0.1% mercuric chloride for 10 minutes. After rinsing with sterile water for 4 times, they were cultured on MS medium without hormone additives at 25°C, and the light intensity was 2000-4000 lux, and sterile seedlings were obtained after 5 days.
[0023] 2) Virus detection: Virus detection is performed on the offspring of asexual seedlings from the same potato tuber, relying on RT-PCR technology, using PVA primer sequences Sense: 5'-TGCCCAGGTATGGTCTTCAACG-3' and Reverse: 5'-ACTTCGGTTACACCCCCCTTCAC-3' to detect Toxicity of test-tube seedlings. The virus detection method was carried out according to the method of X.NIE (X.Nie, R.P.Sing.2001, A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in...
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The invention discloses a method for producing a potato virus A (PVA)-free seedling by utilizing variable temperature virus elimination technology, which belongs to a part of a superior potato breeding system. The method for producing the PVA-free seedling by utilizing the variable temperature virus elimination technology comprises the following steps of: (1) obtaining a tissue culture seedling; (2) performing variable temperature treatment; (3) performing subculture and continuous variable temperature treatment; and (4) performing conventional propagation. In the method, the PVA-free seedling can be obtained by performing the variable temperature test treatment totally for 42 days. The method ensures high PVA-free seedling production efficiency, saves time, labor and energy sources, and promotes the health and sustainable development of potato industry, and is implemented by a simple technical procedure.
Description
technical field [0001] The invention relates to a detoxification technology in a potato detoxification seedling breeding system, in particular to a detoxification technology for producing potato PVA virus-free seedlings by adopting a variable temperature detoxification technology. Background technique [0002] Potatoes are one of the fourth largest food crops in my country. Potatoes are easily infected with a variety of viruses and virus-like viruses during cultivation, and their phenotypes produce complex symptoms such as leaf curling, mosaic leaves, yellowing, and plant rosettes. In agricultural production, potato is mainly expanded through asexual reproduction, and the virus content in the plant can accumulate from generation to generation, resulting in the degradation of viral germplasm resources and a reduction in yield of 40-70%. Due to the increasing worldwide exchange of potato germplasm resources, the worldwide spread of potato viruses has resulted in the worldwide ...
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IPC IPC(8): A01H4/00
Inventor 熊兴耀李炎林何长征刘明月欧阳建文
Owner HUNAN AGRICULTURAL UNIV