Pretreatment method for improving migration capability of mesenchymal stem cells

A MSC and pretreatment technology, which is applied in the field of pretreatment to improve the migration ability of mesenchymal stem cells, can solve the problems of limited autologous serum or plasma, MSC growth stagnation, and inability to meet the needs of MSC expansion, and can improve various The ability to damage the disease, the effect of improving the ability to migrate

Active Publication Date: 2011-04-20
ZHEJIANG UNIV
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Benefits of technology

This technology allows for better cellular immunotherapy by culturing cells outside their blood vessels without any added substances like bovine or horse saliva during surgery procedures. It also enhance how well these cells move into different parts of our bodies when they're taken out from them due to this treatment method.

Problems solved by technology

This patented technical problem addressed in this patents is how to optimize the development process of stem cells derived from placenta during postnatal recovery without causing contamination issues like virus transmission or other factors affecting their quality.

Method used

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  • Pretreatment method for improving migration capability of mesenchymal stem cells
  • Pretreatment method for improving migration capability of mesenchymal stem cells
  • Pretreatment method for improving migration capability of mesenchymal stem cells

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Embodiment 1

[0029] 1. The 10th generation of MSCs derived from menstrual blood (Hangzhou Yiwensai Biotechnology Co., Ltd.) was conventionally cultured in low-sugar DMEM medium (Invitrogen Corporation of the United States) containing 20% ​​FBS (v / v) (Hangzhou Sijiqing Bioengineering Materials Co., Ltd.). ), once pooled, digested and passaged with 0.25% trypsin (w / v)-0.02% ethylenediaminetetraacetic acid (EDTA) (w / v) (Gino Biomedical Technology Co., Ltd.).

[0030] 2. When the menstrual blood MSCs grow to 50% confluence, remove the conventional cell culture medium containing 20% ​​FBS, wash with serum-free and low-sugar DMEM for more than 3 times, and then add 10ng / ml IGF and 10ng / ml PDGF (PeproTech, UK) respectively. ) serum-free N2B27 medium (N2, B27, U.S. Invitrogen Company) (N2, B27 final concentration is 1×, the solvent is low-sugar DMEM medium), or containing 10ng / ml IGF, 10ng / ml bFGF (UK PeproTech Company) Serum-free N2B27 medium (N2, the final concentration of B27 is 1×, the solvent...

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Abstract

The invention provides a pretreatment method for improving the migration capability of mesenchymal stem cells, and the method comprises the following steps: placing the mesenchymal stem cells with stable passage in a serum-free pretreatment culture medium, culturing for 4-5 days, and getting the mesenchymal stem cells after treatment for cellular transplantation, wherein the serum-free pretreatment culture medium comprises 1 multiplied by N2 additive, 1 multiplied by B27 additive, 5-20ng/ml of IGF (insulin-like growth factor), 5-20ng/ml of PDGF (platelet-derived growth factor) or bFGF (basic fibroblast growth factor), and a solvent is low glucose DMEM (Dulbecco minimum essential medium) culture medium or a high glucose DMEM culture medium. By culturing through the serum-free pretreatment culture medium, the cytological features of the MSC (mesenchymal stem cells) can be kept, the migration capability of the MSC after in vivo transplantation can be effectively improved and the capability of treating various injuries and diseases through the transplantation of the MSC can be further improved.

Description

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Claims

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Application Information

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Owner ZHEJIANG UNIV
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