Kit used for detecting susceptibility to femoral head necrosis

A femoral head necrosis and kit technology, applied in the field of kits, can solve problems such as bad living habits and changes, and achieve the effects of high sensitivity, good repeatability, and high throughput

Inactive Publication Date: 2011-06-29
UNION STEMCELL & GENE ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The technical problem to be solved by the present invention is to comprehensively detect and analyze the tested components through a group of test kits related to the susceptibility of femoral head necrosis, using specific primers and probes, and combining single nucleotide extension technology with microarray chip technology. Whether the population carries the "susceptible gene for femoral head necrosis", screen out the susceptible population for femoral head necrosis, change bad living habits, and achieve the purpose of prevention

Method used

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  • Kit used for detecting susceptibility to femoral head necrosis
  • Kit used for detecting susceptibility to femoral head necrosis
  • Kit used for detecting susceptibility to femoral head necrosis

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Embodiment Construction

[0070] The present invention will be described in further detail below in conjunction with specific embodiments. For the scale-up of experiments without specific conditions indicated in the following examples, conventional conditions or the conditions suggested by the manufacturer are generally followed.

[0071] 1 DNA extraction:

[0072] Take the peripheral venous blood of the subject, add the same volume of cell lysate, and lyse twice to fully lyse the white blood cells. After centrifuging and discarding the supernatant, add proteinase K buffer and proteinase K (50ug / ml), incubate at 65°C for 10 minutes, iso Precipitate with propanol, wash twice with 75% ethanol, dry and dissolve in appropriate amount of TB solution.

[0073] 2PCR amplification

[0074] Take the subject's genomic DNA extract and add it to a 96-well plate for multiplex PCR amplification:

[0075] The total reaction volume is 5ul, of which 10M mol / L dNTPs 0.0375ul, 10×PCR Buffer0.5ul, 25mmol / L MgCl 2 1ul...

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Abstract

The invention discloses a kit used for detecting susceptibility to femoral head necrosis. The kit detects six genes which have close relation with the femoral head necrosis, namely a vascular endothelial growth factor (VEGF) gene of vascular endothelial factors, a coagulation factor V (FV) gene of coagulation factors, a methylene tetrahydrofolate reductase (MTHFR) gene, a pamoxonase 1 (PON1) gene of lipid metabolism related gene, an apolipoprotein A1 (APOA1) gene and an apolipoprotein B (APOB) gene. The kit detects a group of genes and loci related to the susceptibility to the femoral head necrosis through specific primers and probes by combining a single nucleotide extension technology with a microarray chip technology, can clearly judge whether examinees carry femoral head necrosis susceptibility genes and screens a group susceptible to the femoral head necrosis from the examinees, so as to make the group change bad living habits and fulfill the aim of preventing the femoral head necrosis.

Description

technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to femoral head necrosis, by detecting single nucleotide polymorphisms of VEGF, FV, MTHFR, APOA1, APOB and PON1 genes locus (SNP) to predict individual susceptibility to femoral head necrosis. Background technique [0002] Femoral head necrosis is often called avascular necrosis of femoral head or aseptic necrosis of femoral head because its main pathology is the blockage of blood supply to the femoral head and the destruction of the head bone caused by ischemia. Femoral head necrosis has a complex pathological process. If it cannot be treated promptly and effectively in the early stage, the femoral head will collapse, the joint space will be narrowed, and finally osteoarthritis will be caused. While suffering from physical pain, patients also suffer from psychological trauma, which also adds a heavy burden to families, units and so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 韩俊领刘志霈周毓玲杜宏伟李楠靳霞刘蓉华
Owner UNION STEMCELL & GENE ENG
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