Kit used for detecting susceptibility to femoral head necrosis

Abstract

The invention discloses a kit used for detecting susceptibility to femoral head necrosis. The kit detects six genes which have close relation with the femoral head necrosis, namely a vascular endothelial growth factor (VEGF) gene of vascular endothelial factors, a coagulation factor V (FV) gene of coagulation factors, a methylene tetrahydrofolate reductase (MTHFR) gene, a pamoxonase 1 (PON1) gene of lipid metabolism related gene, an apolipoprotein A1 (APOA1) gene and an apolipoprotein B (APOB) gene. The kit detects a group of genes and loci related to the susceptibility to the femoral head necrosis through specific primers and probes by combining a single nucleotide extension technology with a microarray chip technology, can clearly judge whether examinees carry femoral head necrosis susceptibility genes and screens a group susceptible to the femoral head necrosis from the examinees, so as to make the group change bad living habits and fulfill the aim of preventing the femoral head necrosis.

Description

technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to femoral head necrosis, by detecting single nucleotide polymorphisms of VEGF, FV, MTHFR, APOA1, APOB and PON1 genes locus (SNP) to predict individual susceptibility to femoral head necrosis. Background technique [0002] Femoral head necrosis is often called avascular necrosis of femoral head or aseptic necrosis of femoral head because its main pathology is the blockage of blood supply to the femoral head and the destruction of the head bone caused by ischemia. Femoral head necrosis has a complex pathological process. If it cannot be treated promptly and effectively in the early stage, the femoral head will collapse, the joint space will be narrowed, and finally osteoarthritis will be caused. While suffering from physical pain, patients also suffer from psychological trauma, which also adds a heavy burden to families, units and so...

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved by the present invention is to comprehensively detect and analyze the tested components through a group of test kits related to the susceptibility of femoral head necrosis, using specific primers and probes, and combining single nucleotide extension technology with microarray chip technology. Whether the population carries the "susceptible gene for femoral head necrosis", screen out the susceptible population for femoral head necrosis, change bad living habits, and achieve the purpose of prevention

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