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Micro-flow control chip and nucleic acid extracting and purifying method

A technology of microfluidic chip and purification method, applied in the field of microfluidic chip and nucleic acid extraction and purification, can solve the problems of low extraction rate, poor repeatability, cumbersome steps, etc., to improve efficiency, simplify manual operation procedures, and improve nucleic acid extraction. The effect of the purification effect

Active Publication Date: 2013-11-06
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method has cumbersome steps, takes a long time, has low extraction rate and poor repeatability

Method used

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  • Micro-flow control chip and nucleic acid extracting and purifying method

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Effect test

Embodiment 1

[0026] Embodiment 1, extraction and purification of human cervical cancer HeLa (Hela) cell nucleic acid:

[0027] A. Use double distilled water and D-Hanks balanced salt solution (D-Hanks balanced salt solution is mainly used to wash cells and tissues, and is also the base liquid for synthetic culture media) to sequentially clean each channel and microunit of the microfluidic chip perform soaking and rinsing;

[0028] B. The cultured human cervical cancer Hela cells were digested and suspended in the culture medium from the adherent culture flask, passed through the first channel 141 into the cell culture microunit 110 of the microfluidic chip, and cultured in a carbon dioxide incubator for 24 Hours, until the cells are covered with the cell culture microunit 110;

[0029] C. Then pass through the first channel 141 into a phosphate buffer solution with a neutral pH (pH value is 7.3-7.5) to wash the cultured cells, and then pass through the digestion solution to detach and dig...

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Abstract

The invention relates to a micro-flow control chip and a nucleic acid extracting and purifying method using same. The micro-flow control chip comprises a cell culture micro-unit, a mixing micro-unit, a nucleic acid collecting micro-unit and a micro-passageway, wherein the cell culture micro-unit is used for cultivating the generative cell and the peptic cell, feeding the peptic cell into the mixing micro-unit for splitting decomposition, feeding the cell lysis solution into the nucleic acid collecting micro-unit, and collecting and purifying nucleic acid; and the micro-passageway is connected with the cell culture micro-unit, the mixing micro-unit and the nucleic acid collecting micro-unit and is the passageway which charges and discharges the liquid. The cell culture, the cell lysis and the nucleic acid collection and the purification are integrated into one micro-flow control chip, so that the extraction and purification working procedure of the nucleic acid can be optimized, the extraction and purification efficiency can be greatly improved, the manual operation procedure can be simplified, the space-time discrimination information which is hardly realized by a conventional method can be realized, and a better nucleic acid extracting and purifying effect can be obtained.

Description

【Technical field】 [0001] The invention relates to the field of nucleic acid extraction and purification, in particular to a microfluidic chip and a nucleic acid extraction and purification method. 【Background technique】 [0002] The separation and purification technology of biomacromolecular ribonucleic acid is the basis for all aspects of molecular biology research and a key technology in life science research and application. With the development and application of microfluidic technology, nucleic acid extraction methods based on microfluidic chips have also been developed. The traditional chip extraction nucleic acid method is to fix silica on the inner wall of the chip, or fill the chip channel with silica gel, diatomaceous earth, glass beads, organosilane particles, magnetic beads and other substrates to adsorb nucleic acid. First, denaturants, proteinase K, etc. were used to lyse the tissue to release the nucleic acid, then extracted with phenol and chloroform, precip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10C12M1/00C12M1/42C07H21/04C07H1/06
CPCC12M47/12C12M35/06C12M47/06C12M29/04C12M27/00
Inventor 龚萍蔡林涛郑明彬胡德红
Owner SHENZHEN INST OF ADVANCED TECH
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