Green and environment-friendly extraction method of stephania tetrandra total alkaloids
A kind of technology of total biological alkaloids, green and environmental protection, applied in the field of extraction of total biological alkaloids of tetrandrol, which can solve the problems of large environmental hazards, complex procedures, large quantities, etc., and achieve the effect of reducing operating procedures, simple process, and easy industrialization
Pending Publication Date: 2022-04-12
科丝美诗(上海)检测科技有限公司 +1
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Problems solved by technology
The existing technology uses a large amount of acid, alkali and organic solvent, which is harmful to the environment, and the process is relatively complicated
[0005] In the patent CN101288695B, the use of an acid aqueous solution is used to infiltrate the coarse powder of Tetradontida first, and then the leachate is absorbed by a macroporous resin column, washed with alcohol and eluted with an ammonia / amine-containing alcohol solution, and the high-purity Tetradontis alkaloid, although Obtained alkaloids with higher purity, but a large amount of waste water will be produced in the process of preparation and extraction
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Embodiment 1
[0047] A green and environment-friendly extraction method of total alkaloids of tetrandrolium, comprising the following steps:
[0048] (1) 500g of tetrandrine (the contents of tetrandrine and fangchinoline obtained through HPLC testing are respectively 1.49%, 0.63%) are pulverized, passed through a 40 mesh sieve, and acidic water (prepared by hydrochloric acid and water) of 4kg pH=3.5 (obtained) was extracted at 85°C for 2 hours, filtered through 5 μm and 0.8 μm polypropylene membranes successively to obtain 3.1 kg of filtrate;
[0049] (2) Add 31g pomegranate tannin to the filtrate, stir evenly, let stand at room temperature for 3h, centrifuge at 8000r / min, and collect 20.3g of solid;
[0050] (3) Disperse the solid in step (2) in 2.0 kg of 30% alcohol, adjust the pH to 4.5-5.0, add 20.3 mg of tannase, and enzymatically hydrolyze at 40-45°C for 3 hours;
[0051] (4) Add 101.5 g of polyamide to step (3), stir and adsorb at room temperature for 1.5 h, filter through a 0.8 μm ...
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Abstract
The invention relates to the field of natural product chemistry, belongs to the IPC classification number of A61K 36/59, and particularly relates to a green and environment-friendly extraction method of stephania tetrandra total alkaloids. The green and environment-friendly extraction method of the stephania tetrandra total alkaloids comprises the following steps: (1) extracting stephania tetrandra root medicinal materials by using acid water, and filtering to obtain filtrate; (2) adding a polyphenol component into the filtrate, uniformly stirring, standing, centrifuging, and collecting a solid; (3) dispersing the solid obtained in the step (2) in 20-40% alcohol, adjusting the pH value to 4.5-5.0, adding an enzyme, and carrying out enzymolysis; and (4) adding polyamide into the solution obtained in the step (3), stirring, adsorbing, filtering, collecting filtrate, and recovering the solvent to obtain the off-white biological total alkali. According to the method, a very good extraction effect can be obtained only by using a small amount of ethanol and hydrochloric acid, and the extraction method is green, environmentally friendly, simple in process and convenient for industrialization.
Description
technical field [0001] The invention relates to the field of natural product chemistry, and belongs to the IPC classification number of A61K 36 / 59, in particular to a green and environment-friendly extraction method of total alkaloids of tetrandrolium. [0002] technical background [0003] The main components of Tetradendronis are alkaloids, mainly tetrandrine and fangchinoline, the total content of which is about 2.0% in Fangji root, and have anti-tumor, analgesic, anti-inflammatory, anti-allergic and anti-hepatic properties. Fibrosis, anti-pulmonary fibrosis, antibacterial, and nervous system protection. [0004] The extraction method of the total alkaloids of Tetradontis tetrandrol is usually acid-water or alcohol-water extraction, the extract is degreased with chloroform and then adjusted to alkaline to precipitate the alkaloids, and then repeatedly acid-adjusted, reduced, recrystallized and refined; or extracted The liquid is adsorbed by the resin, and then eluted with...
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IPC IPC(8): C07D491/18A61K8/49A61K8/9789A61Q19/00
Inventor 周秋娜金荣熙金延俊李华榕胡霞云田星星陈欢
Owner 科丝美诗(上海)检测科技有限公司
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