Method for cultivating ginger-free viruses and cultivating virus-free seedlings by means of propagating and rooting synchronously
A cultivation method and virus-free technology, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of reduced resistance, reduced yield, and quality degradation of ginger, and achieve inhibition of replication, improvement of quality, and metabolic activity. high effect
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[0016] The cultivation method of the present invention will be further described below in conjunction with the examples.
[0017] A kind of ginger virus-free cultivation and virus-free seedling propagation, rooting synchronous culture method, comprises the following steps:
[0018] (1) Selection and disinfection of explants: take the shoots of ginger rhizomes that germinate to a length of 1-2 cm as explants, peel off the outer layer of leaves, soak in aqueous solution of detergent for 5 minutes, rinse with tap water for 15-30 minutes, Disinfect with 0.1% mercury liter for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper; add 2-3 drops of Tween-20 to each liter of 0.1% mercury liter;
[0019] (2) Heat treatment combined with shoot apex tissue culture: the explants obtained in step (1) were treated at 60°C for 15-30 min, placed under a dissecting microscope, and 0.2-0.3 mm of shoot apex meristem was stripped, In...
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