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Method for detecting Beijing genotype of tuberculosis mycobacteria

A technology of Mycobacterium tuberculosis and Beijing genotype, which is applied in the field of genetic engineering, can solve the problems of high cost, complicated operation, and high experimental requirements, and achieve the effects of short detection time, simple operation and low cost.

Inactive Publication Date: 2011-08-17
FUDAN UNIV
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AI Technical Summary

Problems solved by technology

However, it is still time-consuming and labor-intensive when dealing with large samples, and it is impossible to accurately type strains with IS6110 sequence deletion
Combining various typing methods so far, or the sample quality requirements are very high, or the operation is relatively cumbersome, the experiment requires expensive instruments, or the result analysis is heavy and expensive

Method used

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  • Method for detecting Beijing genotype of tuberculosis mycobacteria
  • Method for detecting Beijing genotype of tuberculosis mycobacteria
  • Method for detecting Beijing genotype of tuberculosis mycobacteria

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Embodiment Construction

[0029] Below in conjunction with embodiment, further elaborate this method. It should be understood that these examples are only used to illustrate the method and are not intended to limit the scope of the method. In addition, it should be understood that after reading the content described in this method, those skilled in the art can make various changes or modifications to this method, and these equivalent forms also fall within the scope defined by the appended claims of this application.

[0030] The experimental conditions of the examples all adopt conventional operation methods. The genome extraction of the strains refers to the instructions of the Shanghai Huashun Company’s kit, and the digestion time is slightly modified. Hours were extended to 4 hours to ensure complete lysis of the overthick cell wall of Mycobacterium tuberculosis.

[0031] According to the sequence of each gene on GENEBANK, the sequencing primers were designed by OLIGO 6.0 software. Rv2629 , Rv0...

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Abstract

The invention belongs to the technical field of gentic engineering, in particular to a method for detecting Beijing genotype of tuberculosis mycobacteria. The method comprises the following steps of: detecting mutation of 191 nucleotide A / C of Rv2629 gene and 242 nucleotide G / C of Rv0444c gene by gene amplification and comparison and analysis of sequences, and determining a bacterial strain under test to be the Beijing genotype bacterial strain of tuberculosis mycobacteria if the single-base replacement mutation simultaneously occurs in the Rv2629 gene and the Rv0444c gene. Compared with other methods for distinguishing Beijing genotypes, the method is simple and convenient in operation, has low cost and short detection time and is suitable for rapidly typing tubercle bacillus strains in clinic application or scientific research.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a method for detecting the Beijing genotype of mycobacterium tuberculosis. Background technique [0002] Beijing genotype strains are a group of Mycobacterium tuberculosis with similar genetic background. Such strains are widely distributed and found all over the world. They are a type of strains that spread more rapidly, are more pathogenic, and are easier to form drug resistance. The current definition of the Beijing genotype refers to the fact that the strain has at least three hybridization bands in the spacer 35-43 on the fingerprint of the spacer oligonucleotide typing (Spoligotyping), and there are at least three hybridization bands in the spacer 1-34. Missing Specific Fingerprints. [0003] The method for detecting the Beijing genotype is mainly to use the spacer oligonucleotide typing method to specifically amplify the spacer sequence in the Mycobacterium t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 张鹭王洪海刘鼎乾胡鼎刘岩岩王文婕麦俊滔张敬徐亦薇
Owner FUDAN UNIV
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